用于融合蛋白表达的检测与纯化的表位附加标记技术  

The Detection and Purification of Fused Protein:Epitope Tag

作　　者：马小娟[1] 印莉萍[1] 王丽[1] 

机构地区：[1]首都师范大学生物系遗传与生物工程重点实验室,北京100037

出　　处：《首都师范大学学报（自然科学版）》2004年第4期52-56,共5页Journal of Capital Normal University：Natural Science Edition

摘　　要：简要介绍了表位附加标记的概念、应用、优缺点及其发展前景 .并以c myc标记肽为例 ,简述如何构建用于融合蛋白表达的植物表达载体 ,及如何利用标记肽及其特异性抗体表达并纯化融合蛋白质 .Epitope tagging provides a useful tool for immunological detection,purification and celluar localization of proteins in vivo.This paper described the definition,application,advantages and weaks of epitope tagging briefly.And take c|myc as example,it shows how to construct the eukaryotic expression vector which is used to express the fused protein,and how to detect and purify the fused protein by epitope tagging and its specific monoclonal antibody.

关 键 词：表位 植物表达载体 融合蛋白质 纯化 特异性抗体 C-MYC 标记技术 构建 体表 

分 类 号：R392[医药卫生—免疫学] Q503[医药卫生—基础医学]