细茎石斛多糖DMP2a-1的结构分析  

Structural characterization of the polysaccharide DMP2a-1 from Dendrobium moniliforme

作　　者：徐程[1] 陈云龙[1] 张铭[1] 

机构地区：[1]浙江大学生命科学学院,浙江杭州310012

出　　处：《中国药学杂志》2004年第12期900-902,共3页Chinese Pharmaceutical Journal

基　　金：浙江省自然科学基金资助项目(301560)

摘　　要：目的研究从细茎石斛[Dendrobiummoniliforme(L)Sw]中分离纯化得到的一种相对分子质量为60×103的多糖DMP2a1的化学结构。方法应用紫外、红外、高碘酸氧化、甲基化分析、质谱及1H,13CNMR等化学和光谱方法研究该多糖的结构特征。结果多糖DMP2a1主链由1,4连接的αD吡喃葡萄糖基组成,βD甘露糖以端基糖的形式位于1,4αD吡喃葡萄糖基的O6位上,每6个1,4连接的葡萄糖残基上有1个O6位发生了取代。结论细茎石斛多糖DMP2a1是一新结构多糖,为首次从该植物中分离得到。OBJECTIVE: To investigate the chemical structure of DMP2a-1, a polysaccharide isolated from Dendrobium moniliforme (L.) Sw, with molecular weight of 6 kDa determined by high performance gel permeation chromatography. METHODS: Ultraviolet spectrum, infrared spectrum, periodate oxidation, methylation analysis, mass spectrometry and (1H, 13C) NMR spectrometry were used to determine the structure of DMP2a-1. RESULTS: DMP2a-1 was composed of 4-substituted α-D-glucopyranosyl residues. Mannopyranosyl and glucopyranosyl side chains were present as terminal residue on the 6-position of 4-linked glucopyranosyl residues. Approximately one equivalent of substituted groups per six 4-linked glucopyranosyl residue was present. CONCLUSION: DMP2a-1 is a new polysaccharide and is isolated for the first time from this medicinal plant.

关 键 词：细茎石斛 多糖 结构分析 

分 类 号：R284.2[医药卫生—中药学]