海嘧啶对FC,H_(22)荷瘤小鼠红细胞膜功能的影响  

Effects of Haimiding on the functioning of red cell membrane of FC and H_(22) tumor-bearing mice

作　　者：季宇彬[1] 高世勇[1] 

机构地区：[1]哈尔滨商业大学药物研究所博士后科研工作站,黑龙江哈尔滨150076

出　　处：《中国药学杂志》2004年第12期913-916,共4页Chinese Pharmaceutical Journal

基　　金：国家自然科学基金资助(30271598;30300284);黑龙江省自然科学基金重点项目(ZJY0304);黑龙江省教育厅重大项目基金资助(9551Z006);信息产业部项目(01XK230002);黑龙江省普通高等学校骨干教师创新能力资助计划资助(200101

摘　　要：目的研究海嘧啶对荷瘤小鼠红细胞膜功能的影响。方法采用DPH荧光探针标记的方法测定红细胞膜的流动性,聚丙烯酰胺凝胶电泳法测定红细胞膜蛋白的含量,用比色法测定红细胞膜表面唾液酸含量和膜封闭度。结果海嘧啶能够降低荷瘤小鼠红细胞膜的流动性,降低荷瘤小鼠膜蛋白的含量,升高荷瘤小鼠红细胞膜唾液酸含量,提高红细胞膜重新封闭的能力。结论海嘧啶通过改善和恢复荷瘤小鼠红细胞膜功能,改善机体的免疫功能而达到抗肿瘤的作用。OBJECTIVE: To study the effect of Haimiding on the functioning of red cell membrane of FC and H22 tumor-bearing mice. METHODS: The membrane fluidity of red cells was measured with DPH fluorescence probe as a marker; the amount of red cell membrane proteins was measured using polyacrylamide gel electrophoresis; the amount of sialic acid on the surface of red cell membrane and the sealability of these cells were measured using colorimetric analysis. RESULTS: Haimiding reduced the membrane fluidity of red cells in tumor-bearing mice and the amount of their membrane proteins, while increasing the amount of sialic acid in the membrane of red cells in these mice and enhancing the ability of the membrane of their red cells to reseal. CONCLUSION: The anti-tumor effect of Haimiding on tumor-bearing mice may be due to its ability to improve and restore the functions of the membrane of their red cell and to enhance the immune effect of the organisms.

关 键 词：海嘧啶 荷瘤小鼠 红细胞膜 

分 类 号：R965[医药卫生—药理学] R979.1[医药卫生—药学]