大豆异黄酮类药genistein抑制IL-1β的促破骨细胞骨吸收功能  

Genistein inhibits the promotive effect of IL-1β on osteoclastic bone resorption

作　　者：李斌斌[1] 于世凤[1] 庞淑珍[1] 

机构地区：[1]北京大学口腔医学院病理研究室,北京100081

出　　处：《北京大学学报（医学版）》2004年第6期642-645,共4页Journal of Peking University:Health Sciences

基　　金：国家自然科学基金(30271412)北京大学211工程口腔医学学科群(KQ02211-16)资助~~

摘　　要：目的：观察大豆异黄酮类药 genistein 抑制 IL-1β的促破骨细胞骨吸收功能。方法：新生兔长干骨机械分离法获得破骨细胞(osteoclast．OC)，鼠颅盖骨机械法体外器官培养。按照加入药物的不同分为4组：对照组(不加genistein 和 IL-1β)、10^(-6)mol/L genistein、10μg/L IL-1β、10^(-6) mol/L genistein+10 μg/L IL-1β组。图像分析体外骨吸收陷窝面积、原子分光光度计法测定 OC 培养上清液和器官培养上清液中的 Ca^(2+)含量，用生化法检测器官培养上清液中的酸性磷酸酶(acid phosphatase，ACP)含量。计算实验组与对照组平均值比值，作为骨吸收指数，其值大于1.0表示骨吸收增强，小于1.0表示骨吸收受到抑制。结果：10^(-6)mol/L genistein+10μg/L IL-1β组骨吸收陷窝面积显著高于10^(-6)mol/L gemstein 组，Ca^(2+)含量显著低于10μg/L IL-1β组，骨吸收指数小于10μg/L IL-1β组，大于10^(-6) mol/L genistein 组。器官培养上清液中，各组 ACP 含量差异无显著性；而10^(-6)mol/L genistein+10 μg/L IL-1β组的骨吸收指数低于10μg/L IL-1β组，且二者都大于1.0；10^(-6) mol/L genistein 组的骨吸收指数低于1.0。结论：10^(6)mol/L genistein 和10μg/L IL-1β联合使用显著降低了单独使用 IL-1β导?Objective:To investigate the effect of genistein,a soybean-derived isoflavone,on thestimulating effect on bone resorption of IL-1β.Methods:The osteoclasts(OCs)were isolated with themethods of Yu Shifeng.The rat calvaria were cultured as an organ.The cells in the experiment weregrown in four respectively:Control(without genistein or IL-1β),10^(-6) mol/L genistein,10 μg/L IL-1βand 10^(-6) mol/L genistein+10 μg/L IL-1β.The area of bone resorption,the concentration of Ca^(2+) in thesupernatant liquid of OCs cultures and mice calvaria were tested.The contents of acid phosphatase(ACP)were also examined by biochemistry method.The index of bone resorption was counted as the ra-tio of the experiment average and control ones,which indicated the increase in bone resorption when itwas above 1.0.Results:The area of bone resorption of 10^(-6) mol/L genistein+10 μg/L IL-1β increasedcompared with that of 10^(-6) mol/L genistein,while the concentration of Ca^(2+) in the supernatant liquid ofOCs cultures decreased significantly.The index of bone resorption of 10^(-6) mol/L genistein+10 μg/L IL-1β lied between 10^(-6) mol/L genistein and 10 μg/L IL-1β.In the organ culture,there was no differencein the content of ACP among all the groups,The index of bone resorption of 10^(-6) mol/L genistein+10μg/L IL-1β was below that of 10μg/L IL-1β,but both were above 1.0.The index of bone resorptionwas below 1.0 in the group of 10^(-6) mol/L genistein.Conclusion:Genistein can suppress obviously thebone resorption simulated by single IL-1β.

关 键 词：骨吸收 IL-1Β 破骨细胞 器官培养 对照组 含量 抑制 收功 面积 指数 

分 类 号：R681[医药卫生—骨科学] R780.2[医药卫生—外科学]