肿瘤抗原MAGE-A亚家族HLA-A2限制性CTL表位的预测及分析  

Prediction and analysis of HLA-A2-restricted CTL epitopes derived from the tumor antigen MAGE-A sub-family

作　　者：张秀敏[1] 隋延仿[1] 罗二平[2] 李增山[1] 黄亚渝[1] 司少艳[1] 

机构地区：[1]第四军医大学基础部病理学教研室,陕西西安710033 [2]第四军医大学生物医学工程系军队卫生装备与计量学教研室,陕西西安710033

出　　处：《第四军医大学学报》2004年第24期2222-2225,共4页Journal of the Fourth Military Medical University

基　　金：国家自然科学基金重点资助项目 (39830 4 2 0 )

摘　　要：目的 :预测肿瘤抗原MAGE A亚家族的HLA A2限制性CTL表位 ,为肿瘤治疗选择合适的CTL表位提供依据 .方法 :用SYFPEITHI超基序法远程预测系统和量化基序多项式结合的预测方法进行HLA A2限制性CTL表位预测 ,采用DNAstar软件提供的蛋白质核酸序列编辑、分析工具 ,对MAGE A亚家族成员进行CTL序列同源性分析 .结果 :共预测出了 5 0个HLA A2限制性CTL表位 ,且部分CTL表位高度相似或一致 .结论 :SYFPEITHI超基序法和量化基序多项式法结合的预测方法是一种高效、准确的表位预测方法 ,结合CTL表位的同源性分析 ,可为肿瘤治疗性疫苗的制备中选择合适的CTL表位提供依据 .AIM: To predict the HLA-A2-restricted cytotoxic T lymphocyte (CTL) epitopes of tumor antigen MAGE-A sub-family so as to provide appropriate CTL epitope for tumor immunotherapy. METHODS: The CTL epitopes of tumor antigen MAGE-A sub-family were predicted by SYFPEITHI prediction method combined with the polynomial method. The amino acid sequence and the candidate CTL epitopes of the member of MAGE-A sub-family were analyzed by DNAstar software. RESULTS: Fifty HLA-A2-restricted CTL epitope candidates (nonamers) derived from the tumor antigen MAGE-A sub-family were selected and some epitopes were highly similar or completely identical. CONCLUSION: The combination of SYFPEITHI prediction method and polynomial method can improve the prediction efficiency and accuracy. Analyzing the homology of CTL epitopes and finding common epitopes help to develop some promising antigen peptide tumor vaccines..

关 键 词：抗原 肿瘤 MAGE-A亚家族 T淋巴细胞 细胞毒性 表位 预测 序列分析 

分 类 号：R392.11[医药卫生—免疫学] R394.2[医药卫生—基础医学]