碱性成纤维细胞生长因子对体外培养黑素细胞迁移和[Ca^(2+)]i的影响  

Effects of bFGF on Migration and [Ca^(2+)]i in Human Melanocytes in Vitro

作　　者：张宪旗[1] 冯捷[1] 牟宽厚[2] 马慧群[1] 徐汉卿[1] 牛新武[1] 刘超[1] 党倩丽[1] 

机构地区：[1]西安交通大学第二医院皮肤科,陕西西安710004 [2]西安交通大学第一医院皮肤科,陕西西安710061

出　　处：《中国皮肤性病学杂志》2004年第12期708-710,共3页The Chinese Journal of Dermatovenereology

基　　金：国家自然科学基金资助 (30 2 0 0 2 4 7)

摘　　要：目的　探讨碱性成纤维细胞生长因子 (bFGF)对体外培养黑素细胞迁移和细胞内游离钙离子浓度([Ca2 + ]i)的影响。方法　从正常人包皮分离、纯培养黑素细胞 ,用bFGF处理纯培养的黑素细胞 ,采用Transwell微孔膜法研究黑素细胞迁移 ,采用激光共聚焦扫描显微镜检测 [Ca2 + ]i。结果　bFGF可促使黑素细胞迁移 ,并诱导 [Ca2 + ]i升高。结论　bFGF可能通过诱导黑素细胞 [Ca2 + ]i升高从而促进黑素细胞的迁移。Objective To observe the effects of bFGF on human melanocytes migration and [Ca 2+ ]i in vitro. Methods Human melanocytes were obtained from normal foreskins and motility was assessed using the Transwell micropore filter method.Laser confocal microscope was employed to measure [Ca 2+ ]i.Results bFGF could stimulate melanocytes migration through micropore filter and increase [Ca 2+ ]i.Conclusion It is suggested that bFGF might enhance melanocytes migration by increasing the [Ca 2+ ]i of melanocyte.

关 键 词：黑素细胞 碱性成纤维细胞生长因子 迁移 钙离子 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]