家蚕胚胎发育关联的初始蛋白质研究  

Analysis of Characterization of the Key Proteins Associated Embryonic Development of the Silkworm,Bombyx mori

作　　者：姚国华[1] 钟伯雄[1] 颜新培[1] 沈飞英[1] 陈齐龙[1] 周丽[1] 童小芬[1] 

机构地区：[1]浙江大学动物科学学院生物资源科学系,杭州310029

出　　处：《蚕业科学》2004年第4期436-439,共4页ACTA SERICOLOGICA SINICA

基　　金：国家自然科学基金 (编号 3 0 2 710 0 4)

摘　　要：以人工孵化卵、滞育卵和未受精卵为材料 ,通过蛋白质双向电泳分析和图谱比较分析 ,发现受精后的人工孵化卵和滞育卵 ,第 2、4、5和 6号蛋白斑点的表达量较未受精卵有所增加 ,人工孵化处理后的蚕卵第 2、3号蛋白斑点的表达量较滞育卵有所增加。推测这几种蛋白质可能在促使受精过程的完成以及促使胚胎解除滞育进入生长发育阶段方面发挥作用。Using the artificial hatched eggs,diapause eggs and unfertilized eggs as material,it were detected that the increased expression of the 2nd,4th,5th and 6th protein spots after the fertilization,and the 2nd and 3rd protein spots after the artificial hatch by the protein two-dimensional polyacryamide gel electrophoresis (2D-PAGE) and image analysis.These results suggest that the changes of these proteins maybe have great effect on the promotion of the fertilization and the breaking of the embryo diapause to development.

关 键 词：家蚕 蚕卵 蛋白质电泳 图谱分析 胚胎发育 

分 类 号：S881.2[农业科学—特种经济动物饲养] Q51[农业科学—畜牧兽医]