丁硫氨酸亚砜胺体外逆转人膀胱癌细胞多药耐药性的研究  

Study for in vitro reversing multidrug resistance on human bladder cancer cells by buthionine sulfoximine

作　　者：曾晓勇[1] 叶章群[1] 杨为民[1] 陈忠[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院泌尿外科,武汉430030

出　　处：《临床泌尿外科杂志》2005年第1期38-40,共3页Journal of Clinical Urology

基　　金：湖北省自然科学基金项目(No 99J124)

摘　　要：　　　目的:探讨丁硫氨酸亚砜胺(BSO)对体外培养的人膀胱癌细胞株多药耐药性的影响。方法:以 20μmol/L的BSO处理人膀胱癌细胞株BIU87及其多药耐药亚株BIU87/A后约18 h,分别应用定量逆转录多聚酶链式反应法(RT PCR)、荧光分光光度法及MTT法,检测两株细胞γ 谷氨酰半胱氨酸合成酶(γ GCSh)mRNA的表达和细胞内谷胱甘肽(GSH)水平,以及细胞对多种药物敏感性的变化。结果:BSO处理后,BIU87/A 细胞γ GCSh mRNA的表达水平为1.091,明显低于处理前的 1.534(P<0.01)。与之对应,细胞内GSH浓度也由处理前的 177.3 mmol/g蛋白质降至处理后的 89.0 mmol/g蛋白质(P<0.05)。BSO处理使 BIU87/A细胞对大部分化疗药物的敏感性增高。结论:BSO可通过抑制细胞的GSH的合成,使膀胱癌耐药细胞的化疗耐药性得到逆转。Objective:To determine the effect of buthionine sulfoximine(BSO) in vitro on multidrug resistance (MDR) in human bladder cancer cells.Methods:Human bladder cancers cell lines BIU87 and it`s durg resistance subline BIU87/A were exposed in 20 μm BSO for 18 h and then the expression of g-GCSh mRNA, intracellular glutathione(GSH) levels and the sensitivity of both cell lines to chemotherapy agents were measured respectively by the quantitative reverse transcription polymerase chain reaction(RT-PCR), fluoro-spectrophotography and MTT assay before and after BSO exposure.Results:In compared with before BSO exposure, both the expression of g-GCSh mRNA and the intracellular GSH content of BIU87/A after BSO exposure are decreased significantly ( 1.534 vs 1.091, P< 0.01; 177.3 μmol/mg protein vs 89.0 μmol/mg protein). The sensitivity of BIU87/A to most of chemotherapy agents increased significantly after BSO esposure.Conclusions:BSO can reverse in vitro the drug resistance in bladder cancer MDR cells by reducing the content of intracellular GSH.

关 键 词：丁硫氨酸亚砜胺 膀胱肿瘤 耐药性 抗肿瘤药 

分 类 号：R694[医药卫生—泌尿科学]