龙葵碱对H_(22)荷瘤小鼠肿瘤细胞膜唾液酸和封闭度的影响  

Effect of Solanum nigrum total alkaloid on sialic acid and blocking degree of tumor cell membrane in H_(22) tumor-bearing mice

作　　者：季宇彬[1] 王胜惠[1] 高世勇[1] 邹翔[1] 汲晨峰[1] 

机构地区：[1]哈尔滨商业大学药物研究所博士后科研工作站,黑龙江哈尔滨150076

出　　处：《中草药》2005年第1期79-81,共3页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(30400591);黑龙江省自然科学基金资助项目(D2004-13);哈尔滨市青年科学基金资助项目(2004AFQXJ035)。

摘　　要：目的研究龙葵碱对H22荷瘤小鼠肿瘤细胞膜唾液酸(SA)水平和封闭度的影响。方法建立H22荷瘤小鼠模型,荷瘤小鼠分别sc生理盐水、环磷酰胺、龙葵碱(9.375、18.75、37.5mg/kg)。采用比色法测定H22小鼠肿瘤细胞膜SA水平和封闭度。结果龙葵碱可剂量依赖性地降低荷瘤小鼠肿瘤细胞膜SA水平和肿瘤细胞膜封闭度。结论龙葵碱通过降低H22荷瘤小鼠肿瘤细胞膜SA水平和肿瘤细胞膜封闭度起抗肿瘤作用。Objective To study the effects of total alkaloid from Solanum nigrum (TASN) on sialic acid (SA) and blocking degree of tumor cell membrane in H_(22) tumor-bearing mice. Methods H_(22) tumor-bearing mice model was established and H_(22) tumor-bearing mice were given NS, Cytoxan (CTX), and TASN (9.375, 18.75, and 37.5 mg/kg) by sc, respectively. SA content and blocking degree of tumor cell membrane in H_(22) mice were determined by chromatometry. Results TASN could decrease the SA content and the blocking degree of tumor cell membrane in H_(22) tumor-bearing mice in a dose-dependent manner. Conclusion TASN shows the antitumor activity through decreasing the SA content and blocking degree of tumor cell membrane in H_(22) tumor-bearing mice.

关 键 词：龙葵碱 H22荷瘤小鼠 膜封闭度 唾液酸 

分 类 号：R286.91[医药卫生—中药学]