正品龙胆遗传多样性的RAPD及ISSR分析  

Analysis of genetic diversity in certified Radix Gentianae by RAPD and ISSR

作　　者：曹雅男[1] 李庆章[1] 孙岳[1] 李璐[1] 王大威[1] 李景鹏[1] 

机构地区：[1]东北农业大学生命科学学院,黑龙江哈尔滨150070

出　　处：《中草药》2005年第1期100-103,共4页Chinese Traditional and Herbal Drugs

基　　金：黑龙江省教委重大资助项目(9551Z002)

摘　　要：目的用RAPD和ISSR方法分析了4种正品龙胆的亲缘关系及遗传多样性,为正品龙胆的品种鉴定及栽培育种提供了分子生物学依据。方法优化RAPD及ISSR的PCR反应体系,对产物琼脂糖凝胶电泳结果进行数据统计。结果从100个RAPD引物和32个ISSR引物中分别筛选出10个RAPD引物和4个ISSR引物。通过遗传距离系数UPGMA聚类法分析数据,构建类聚关系树系图。结论RAPD及ISSR的PCR反应体系可以获得理想的实验结果,适用于龙胆品种遗传多样性及亲缘关系的分析。Objective To analyze the sibling relationship and genetic diversity of four certified Radix Gentianae (RG) species by RAPD and ISSR methods. It provided molecular biological proof for species identification and breeding of certified RG. Methods PCR reaction systems of RAPD and ISSR were optimized, and the agarose gel electrophoresis results were analyzed into statistic data. Results Ten RAPD primers and four ISSR primers were selected respectively from 100 RAPD primers and 32 ISSR primers. The statistic data were analyzed to construct cluster analysis dendrogram by genetic distance UPGMA method. Conclusion The PCR reaction systems of this experiment gets ideal RAPD and ISSR results that suit to the analysis of certified RG species in genetic diversity and sibling relationship.

关 键 词：RAPD 遗传多样性 ISSR分析 龙胆 亲缘关系 PCR反应体系 品种鉴定 正品 琼脂糖凝胶电泳 分子生物学 

分 类 号：R282[医药卫生—中药学] S567[医药卫生—中医学]