α-促黑素细胞激素对瘢痕疙瘩成纤维细胞分泌转化生长因子-β1的影响  

Effects of α-melanocyte-stimulating hormone on TGF-β_1 secretion of fibroblasts from keloids

作　　者：郑健生[1] 邢新[2] 张敬德[2] 

机构地区：[1]解放军第一七五医院烧伤整形科,福建漳州363000 [2]第二军医大学长海医院整形外科,上海200433

出　　处：《医学研究生学报》2005年第1期34-36,共3页Journal of Medical Postgraduates

摘　　要：目的 :研究α 促黑素细胞激素 (α MSH)对体外培养的瘢痕疙瘩成纤维细胞分泌转化生长因子 β1(TGF β1)的影响 ,为研究瘢痕疙瘩的病因提供新的依据。　方法 :取人的瘢痕疙瘩组织 ,利用组织块培养法进行成纤维细胞原代培养 ,DMEM培养液传代、扩增培养 ,加入 10 -6mmol/L浓度的α MSH ,应用ELISA方法检测其水平 ,分析α MSH对人瘢痕疙瘩成纤维细胞分泌TGF β1的影响。　结果 :10 -6mmol/L浓度的α MSH可明显促进瘢痕疙瘩成纤维细胞TGF β1的分泌。　结论 :一定剂量的α MSH对体外培养的人瘢痕疙瘩成纤维细胞TGF β1的分泌具有促进作用。Objective: To investigate the effects of α-melanocyte-stimulating hormone(α-MSH) on TGF-β_1 secretion of fibroblasts from keloids so as to find a novel evidence to research etiology of keloids.Methods:Fibroblasts isolated from human keloids were cultured in DMEM medium with 15% bovine serum and treated with α-MSH. The level of TGF-β_1 was measured with ELISA method. Results: α-MSH at the concentration of 10 -6 mmol/L triggered the TGF-β_1 secretion of fibroblasts. Conclusion:α-MSH possesses the effect of triggering human keloid fibroblasts to secrete TGF-β_1, and its effective concentration is 10 -6mmol/L.

关 键 词：Α-促黑素细胞激素 瘢痕疙瘩 成纤维细胞 转化生长因子-Β1 

分 类 号：R392.11[医药卫生—免疫学]