骨髓单个核细胞缺血肢体局部移植对缺血组织血管再生作用的研究  

Augmentation of Neovascularization by Implantation of Autologous Bone Marrow Mononuclear Cells in a Rat Ischemic Limb Model

作　　者：刘文亮[1] 尹邦良[1] 喻风雷[1] 胡野荣[1] 陈晓凤[1] 

机构地区：[1]中南大学湘雅二医院胸心外科,湖南长沙410011

出　　处：《中国医师杂志》2005年第1期72-74,共3页Journal of Chinese Physician

摘　　要：目的　分析骨髓单个核细胞局部移植对缺血组织血管再生的作用及机制。方法　从大鼠胫骨及股骨采集骨髓细胞 ,密度梯度离心法分离出骨髓单个核细胞。分离、切断近交系Wistar大鼠右侧股动脉及其分支 ,建立单侧后肢缺血模型。分 7点局部注射单个核细胞悬液 (移植组 )或磷酸盐缓冲液 (对照组 )。术后第 3、7、14d获取内收肌制作组织匀浆 ,ELISA法测定匀浆中bFGF、VEGF和IL -1β因子水平 ;术后第 2、4周免疫组化法分析肌肉组织毛细血管密度。 结果　术后第 3d ,移植组bFGF表达水平高于对照组 (P <0 0 1)。术后第 3、7d ,移植组IL -1β水平高于对照组 (P <0 0 1)。术后第 2、4周移植组内收肌毛细血管密度高于对照组(P <0 0 1)。结论　在大鼠缺血后肢局部直接植入骨髓单个核细胞可诱导新生血管形成 ;骨髓单个核细胞分泌bFGF、IL -1β等多种因子在局部直接植入骨髓单个核细胞促进血管再生的机制中可能起主要作用。Objective To investigate the effect of autologous bone marrow mononuclear cells (BM-MNCs) implantation on neovascularization in rat model of hind limb ischemia, and explore its mechanism. Methods Rat BM-MNCs were isolated from tibia and femur using density gradient centrifugation. A rat model of ischemic hind limb was made by the ligation of the right femoral artery and its branches in imbred Wistar rats. BM-MNCs were injected into 7 points of the ischemic muscles (20μl/point). The levels of VEGF, bFGF and IL-1β in the ischemic musles at the third, 7th and 14th days after ligation were measured by ELISA. Micro-vessel density (MVD) in the ischemic mulsles was evaluated at the second and 4th weeks after ligation. Results The levels of bFGF and IL-1β in the implantation group were significantly higher than those in the control group. The MVD of ischemic mulsles in the implantation group was also significantly higher than that in the control group at the second and 4th weeks after treatment. Conclusion The implantation of autologous BM-MNCs could induce neovascularization in a rat ischemic hind limb, and bFGF and IL-1β secreted by BM-MNCs might play a role in the neovascularization.

关 键 词：骨髓 单个核细胞 移植 血管再生 缺血 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]