聚酰胺-胺(PAMAM)树状大分子对甲氨蝶呤的包合及缓释研究  

Complex and in vitro Release of Methotrexate-PAMAM Dendrimer

作　　者：叶玲[1] 江晓舟[1] 杨华[1] 苏健婷[1] 恽榴红[2] 

机构地区：[1]首都医科大学化学生物学与药学院,北京100054 [2]军事医学科学院毒物药物研究所,北京100850

出　　处：《高等学校化学学报》2005年第2期353-355,共3页Chemical Journal of Chinese Universities

基　　金：北京市科技新星 (批准号 :95 4812 0 0 0 );北京市教委科技发展计划项目 (批准号 :0 0 KJ-10 7)资助 .

摘　　要：以甲氨蝶呤 (MTX)为模型药物 ,研究了 G5 .0 PAMAM树状大分子对其包合和释放 ,并用 1 3C NMR对 PAMAM-MTX包合物进行了表征 .UV-Vis研究结果表明 ,1个 G5 .0 PAMAM树状大分子能包合 2 7个MTX分子 ,体外释放研究表明 ,在 3 7℃ ,p H=7.4的 1 0 mmol/LTris-HCl缓冲溶液中 G5 .0 PAMAM树状大分子对 MTX具有明显的缓释作用 .We investigated the interaction between G5\^0 PAMAM and methotrexate(MTX) drug molecules by 13C NMR, and it has been proven that the interaction is occurring between the PAMAM amine functional groups and the MTX carboxyl groups. UV-Vis study showed each G5.0 PAMAM could conjugate up to 27 MTX molecules. The release of MTX from complex is much slower than that of free MTX at pH=7.4, 10 mmol/L Tris-HCl buffer. The presence of 0.02 mol/L NaCl in buffer increases the release rate of MTX from the complex, which further defines the interaction between PAMAM and MTX.

关 键 词：PAMAM树状大分子 甲氨蝶呤(MTX) 包合 缓释 

分 类 号：R91[医药卫生—药学]