金华猪肌细胞生成素基因的初步研究  

Primary Investigation on MyoG Gene in Jinhua Pig

作　　者：赵进[1] 聂光军[2] 张金枝[1] 郭晓令[1] 徐宁迎[1] 

机构地区：[1]浙江大学动物科学学院,浙江杭州310029 [2]安徽工程科技学院,安徽芜湖241000

出　　处：《养猪》2005年第1期23-24,共2页Swine Production

基　　金：国家"973"(编号:G2000016104);"863"(编号:2001AA213121)资助项目

摘　　要：运用PCR-RFLP方法对金华猪I系(178头)、II系(30头)、III系(41头)的肌细胞生成素(MyoG)基因的第2内含子内(PCR1-MspⅠ-RFLP)和3'端(PCR2-MspⅠ-RFLP)位点进行多态性分析。结果表明,PCR1-MspⅠ-RFLP位点有3种基因型,即AA型、AB型和BB型,其基因型频率分别为0.1544、0.3826、0.4631;PCR2-MspⅠ-RFLP位点只有2种基因型,MM型和MN型,其基因型频率分别为0.9953和0.0047。The genotypes of myogenin gene(at second intron and at 3' end)for 249 Jinhua pigs(line I,178;line II, 30;line III,41) were detected by PCR-RFLP. There were three genotypes of AA,AB and BB in the PCR1- MspⅠ-RFLP site,their frequencies were 0.154 4、0.382 6、0.463 1 respectively; Genotypes only were MM and MN in PCR2- MspⅠ-RFLP site,and the frequencies were 0.995 3 and 0.004 7.

关 键 词：基因型频率 PCR—RFLP方法 肌细胞生成素 初步研究 MM AB型 AA 金华猪 位点 BB 

分 类 号：S828.2[农业科学—畜牧学]