二肽在断奶仔猪小肠刷状缘膜囊中水解状况的研究  

Hydrolysis of Dipeptides in Small Intestinal Brush Border Membrane Vesicles of Weaned Piglets

作　　者：代建国[1] 李德发[1] 朴香淑[1] 潘宝海[1] 龚利敏[1] 

机构地区：[1]中国农业大学动物科技学院,北京100094

出　　处：《畜牧兽医学报》2005年第2期133-136,共4页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金项目(39870583)

摘　　要：通过孵育试验研究了甘- L- 亮(Gly -Leu)和- L- 组 L- 亮(His- Leu) 2 种二肽在断奶仔猪小肠刷状缘膜囊(Brush Border Memlorane Vesides,BBMV)中的水解状况。结果表明,孵育液 pH值(分别为 3.5、4.0、4.5、5.0、5 5、6.0、6.5、7.0、7.5和8.0)、孵育温度(25 ℃和 37 ℃)、孵育时间(0 5、1、2、5、10、20、30 和 40 min)以及 BBMV(二肽酶)的添加量均不影响此 2 种二肽在断奶仔猪小肠 BBMV孵育体系中的水解。结果提示,Gly- Leu和 His- Leu 2种二肽在断奶仔猪小肠BBMV体系中均不水解。In vitro experiments were carried out to investigate the effects of incubation solution pH, incubation temperature, incubation time and the addition amount of BBMV (presumably dipeptidase) on hydrolysis of dipeptides. The results indicated that incubation solution pH (3.5,4.0,4.5,5.0,5.5,6.0,6.5,7.0,7.5 and 8.0) , incubation temperature (25 ℃ and 37 ℃) , incubation time (0.5, 1, 2, 5, 10, 20, 30 and 40 min) and the addition amount of BBMV were not effective on hydrolysis of the dipeptides. The results suggested that these two dipeptides (Gly-Leu and His-Leu) were not hydrolyzed in small intestinal BBMV of weaned piglets.

关 键 词：断奶仔猪 刷状缘膜囊 孵育液 小肠 添加量 温度 PH值 二肽 状况 影响 

分 类 号：S828[农业科学—畜牧学] R446[农业科学—畜牧兽医]