伊氏锥虫多种克隆群体的采集及血清学检测  

Collection of Clone-derived Populations of Trypanosoma evansi Stock YN and Determination of its Variable Antigenic Types by IFT and ABC-ELA

作　　者：栗利芳[1] 袁志波[1] 索勋[1] 汪明[1] 孔繁瑶[1] 

机构地区：[1]中国农业大学动物医学院,北京100094

出　　处：《畜牧兽医学报》2005年第2期198-201,共4页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金资助项目(30070573; 30270991)

摘　　要：针对伊氏锥虫抗原变异,国内外学者对抗原变异规律进行了研究,对其抗原变异程序、变异数量、变异频率等有了一定的了解,在此基础上进行免疫学研究进而寻求防治锥虫病的免疫学手段[1～4].但多局限于某种动物个体感染锥虫后的一小段有限的时间进行,所获得的抗原变异型数量较少[1,5],因而很难澄清如:一个虫株究竟能有多少变异机会,是以怎样的程序变异的等问题.本试验采集一个锥虫克隆群体在新西兰大白兔体内的连续抗原变异体,通过对所得抗原变异体的检测,进一步探索伊氏锥虫的抗原变异规律.In order to find out the regularity of antigenic variation, 10~7 cloned population of Trypanosoma evansi stock YN was inoculated sequentially into 5 New Zealand rabbits during 165 days. Blood was collected from the rabbits at 3-day intervals after infection. Ten clone-derived populations were isolated from each; twenty VATs were determined from the 50 populations with the indirect fluorescent antibody staining technique (IFT) and ABC-enzyme-linked assay (ABC-ELA) and designated as BeTat 1.1, BeTat 1.2,…BeTat 1.20.

关 键 词：伊氏锥虫 抗原变异 锥虫病 群体 数量 变异频率 采集 血清学检测 免疫学研究 感染 

分 类 号：S852[农业科学—基础兽医学] R373[农业科学—兽医学]