各种胆红素对氧化酶法测定血清尿酸浓度的影响  

The influence of different kinds of bilirubin on the direct enzymatic measurement of uric acid

作　　者：王缚鲲[1] 王宪灵[1] 安黎云[1] 张宏[1] 王薇[1] 李玮[1,2,3,4] 

机构地区：[1]解放军白求恩国际和平医院检验科,河北石家庄050082 [2]结合胆红素 [3]未结合胆红素 [4]δ胆红素

出　　处：《检验医学》2005年第1期4-6,共3页Laboratory Medicine

摘　　要：目的　通过分析不同胆红素对尿酸(UA)氧化酶方法的干扰,探讨各种胆红素对过氧化物酶偶联Trinder反应的UA氧化酶测定方法的影响。方法　我们制备了24种含有不同浓度UA的血清,每种血清含有不同浓度的未结合胆红素(Bu)、结合胆红素(Bc)、δ胆红素(δBil),使用美国EKTACHE750XRCanalyzer生化分析仪测定各种胆红素浓度,使用日本7170全自动生化分析仪测定血清中的UA浓度。结果　来自Bc的干扰最大,当浓度为400μmol/L时,回收率只有56.4%;Bu的干扰较小,当浓度400μmol/L时,回收率仍达87.4%;δBil的干扰最小,随着血清中δBil浓度的提高,对UA测定基本无影响,回收率保持在93.4%以上。结论　Bu、Bc、δBil对UA氧化酶测定方法影响程度不同,在实际工作中应予以重视。Objective To examine the possbile effect of different kinds of bilirubin on the direct enzymatic measurement of uric acid(UA) by using the uricase and peroxidase coupled reaction. Methods We perpared twenty four sera containing different levels of UA, each contained a different concentration of unconjugated bilirubin(Bu), conjugated bilirubin (Bc) and delta bilirubin(δ-Bil). The concentration of Bu, Bc and δ-Bil was measured by EKTACHE 750XRC analyzer, and the concentration of UA was measured by HITACHI 7170 automatic analyzer. Results Bc produced greatest negative interference, when concentration of Bc was 400 μmol/L, recovery rate of UA was only 56.4%. The effect of Bu was slight, when concentration was 400 μmol/L, recovery rate of UA was 87.4%. Interference by δ-Bil was less than that by Bu. Conclusions Different kinds of bilirubin produce negative interference in the measurement of UA. The interference of Bc is greater than Bu, the interference of δ-Bil is slightest.

关 键 词：UA Δ胆红素 血清尿酸 氧化酶法 影响 TRINDER反应 胆红素浓度 酸浓度 过氧化物酶 干扰 

分 类 号：R541.4[医药卫生—心血管疾病] R446[医药卫生—内科学]