CHROMager显色培养基分离沙门菌的初步应用和评估  

Primary application and evaluation of CHROMagar salmonella medium and WS medium for isolation of Salmonellae from stool samples

作　　者：杨兰萍[1] 许学斌[2] 姚宗蓓[1] 顾宝柯[2] 

机构地区：[1]上海市卢湾区疾病预防控制中心,上海200025 [2]上海市疾病预防控制中心,上海200336

出　　处：《检验医学》2005年第1期52-54,共3页Laboratory Medicine

基　　金：上海市卢湾区卫生局课题基金资助项目(卢2002 14)

摘　　要：　　目的　初步评估CHROMagar 显色培养基(CAS)检测肛拭中沙门菌的效果。方法　以实验室保存沙 门菌和非沙门菌作为测试菌共190株,测试CAS、武汉SS平板(WS)上细菌生长的敏感性、特异性;并应用CAS 与WS对1717份餐饮业人员肛拭作沙门菌的现场分离效果比较。结果　CAS对测试菌和现场检测的敏感性均 为100%,明显优于WS的57.14%和31.58%;两者在肛拭样品中沙门菌检出率分别为1.11%(19/1717)和 0.35%(6/1717)。结论　CAS平板具有敏感性高、检出率高、易于掌握等特点,建议作为粪便常规分离或鉴别沙 门菌的首选培养基。Objective To evaluate the characteristics of CHROMagar salmonella medium (CAS) for isolation of Salmonellae from stool samples. Methods Total 190 strains of Salmonella and non-Salmonella from laboratory stock isolates were inoculated on CAS and modificatory SS(WS) plates to test their sensitivity and specificity. Anus wiping samples from 1 717 employees of food related units were tested for Salmonellae by CAS and WS medium, and results between two tests were compared. Results The sensitivity in stock strains testing and samples isolating of CAS broth was 100% and much better than that of WS(57.14%, 31.58%). The positive rates of anus wiping test in CAS and WS were 1.11%(19/1 717) and 0.35%(61/1 717). Conclusions CAS plate is more sensitive and detective than traditional WS plate and easier to operate. It suggests that CAS plate should be the first choice in routine detection and distinguishing Salmonellae of stool specimens.

关 键 词：沙门菌 显色培养基 检出率 敏感性 CAS 评估 分离 细菌生长 特异性 平板 

分 类 号：R378.22[医药卫生—病原生物学] R155[医药卫生—基础医学]