高压芒刺静电场对人肝癌细胞SMMC7721生长的影响  

Effects of High-voltage Prickle Electrostatic Field on the Growth of Human Hepatocarcinoma Cell SMMC7721

作　　者：孙迎春[1] 严羚玮[1] 金光辉[2] 张羽[1] 刘扬[2] 

机构地区：[1]东北师范大学物理学院,吉林长春130024 [2]吉林大学卫生部放射生物重点实验室,吉林长春130021

出　　处：《中国医学物理学杂志》2005年第1期391-392,共2页Chinese Journal of Medical Physics

基　　金：吉林省科技发展计划项目(20030543-3);教育部留学回国人员科研启动基金(2002)

摘　　要：目的:研究高压芒刺静电场对肿瘤细胞生长的影响及杀伤作用。方法:用不同强度高压芒刺静电场作用于体外培养的人肝癌细胞SMMC7721,分别用MTT比色法和流式细胞术检测电场处理后细胞的增殖能力和死亡率。结果:处理后细胞的增殖受到明显的抑制(P<0.05),且细胞的死亡率均显著高于对照组(P<0.01),其中14kV组细胞的死亡率与对照组相比增加了173.7%。结论:一定强度的高压芒刺静电场能够有效抑制肿瘤细胞的生长,并具有一定的杀伤作用。Objective: To investigate the effects of high-voltage prickle electrostatic field (HVPESF)on proliferation of tumor cell and killing effect. Methods: Human hepatocarcinoma cell SMMC7721 was treated by different intensities of HVPESF. The MTT method and flow cytomelry were adopted to delect the proliferation and the mortality rale of cell SMMC7721, respectively. Results: The cell proliferation was inhibited significantly (P<0.05)and the cell mortality rates were significantly higher than one of control group (P<0.01). The mortality rale 14 kV group increased to 173.7% of control group. Conclusion: A certaim intensities of HVPESF can inhibit growth of tumor cells and kill tumor cells.

关 键 词：高压芒刺静电场 人肝癌细胞SMMC7721 MTT 流式细胞术 

分 类 号：Q64[生物学—生物物理学]