贵州黑山羊遗传结构的RAPD分析  

RAPD analysis on genetic construction of Guizhou black goat

作　　者：陈祥[1] 廖正录[2] 张勇[1] 张芸[2] 李国红[3] 简承松[1] 魏泓[4] 

机构地区：[1]贵州大学动物科学学院,贵州贵阳550025 [2]贵州省农业厅畜禽品种改良站,贵州贵阳550001 [3]贵州师范大学生物系,贵州贵阳550001 [4]第三军医大学实验动物中心,重庆400038

出　　处：《四川畜牧兽医》2005年第2期25-26,共2页Sichuan Animal & Veterinary Sciences

基　　金：贵州大学校基金(2000);贵州省科学技术基金犤黔基合计字(2002)305号犦;国家自然科学基金(30070120)项目资助。

摘　　要：用27条多态性引物对15份贵州黑山羊个体基因组进行RAPD分析。结果共扩增出258条带,其中多态带为119条,多态频率在0～77.78%之间,平均多态频率为46.12%。单个引物获得的扩增带数在4～14条之间,平均每条引物扩增出9.56条带。扩增带分子量范围为230bp～2800bp。用Nei氏公式计算品种内个体间的遗传相似性指数平均为0.9023。结果表明:贵州黑山羊个体间的遗传变异较大,具有较丰富的遗传结构。In this paper,27 polymorphic primers were used to amplify the genome DNA of 15 Guizhou black goats. After the amplification,258 bands were found,and the polymorphic bands were 119 with the frequency of 0～77.78%(mean 46.12%). Band number was between 4 and 14(mean 9.56), and the fragment length of products was between 230 bp and 2800 bp in every primer. The genetic similarity index among individuals were calculated by Nei., it was 0.9156. The results displayed that Guizhou block goats'genetic variation was higher and the genetic construction among the individuals was abundant.

关 键 词：黑山羊 RAPD分析 遗传结构 引物 遗传变异 遗传相似性 品种 扩增 多态性 基因组 

分 类 号：S827.2[农业科学—畜牧学]