利用土壤筛选紫花苜蓿高效共生根瘤菌的初步研究  

Screening of high efficient symbiotic rhizobial strains of Alfalfa by soil test

作　　者：刘杰[1] 王赟文[2] 李颖[1] 汤辉[1] 汪玲玲[1] 

机构地区：[1]中国农业大学生物学院微生物系,北京100094 [2]中国农业大学动物科技学院草业科学系,北京100094

出　　处：《草业科学》2005年第2期21-25,共5页Pratacultural Science

基　　金：国家重点基础研究发展计划资助子项目(2001CB108905)

摘　　要：采用西北地区土壤试管栽培法对12株不同地区来源的根瘤菌和2个紫花苜蓿Medicago sativa品种(品系)进行接种效果的研究,旨在使筛选结果尽量接近实际生产环境,筛选出符合实际效果的高效共生菌株.结果表明,紫花苜蓿与根瘤菌之间表现出共生效果的多样性.绝大多数接种根瘤菌的紫花苜蓿在总瘤数、有效瘤数、地上部干质量方面与不接种的对照相比分别增加了7%～240%,10%～367%,7%～150%.根瘤菌与紫花苜蓿品种之间存在着互作关系,且受土壤因子尤其是土壤养分状况的影响.初步筛选出的与2个紫花苜蓿品种(品系)最佳共生匹配的根瘤菌菌株分别是: 中苜一号的73317,01006,01055;金皇后的83092,01006,01055.The effects of inoculation with 12 rhizobial strains on two Medicago sativacultivars were conducted by soil test, aimed at screening the efficient symbiotic strains for each cultivars under a condition closed to the field. The results showed that most of rhizobial strains promoted the plant growth of both cultivars. Compared with the controls, the numbers of total nodules of alfalfa plants inoculated with rhizobium increased by 7%~240%, the numbers of effective nodules increased by 10%~367%, and the aboveground dry weight increased by 7%~150%. Significant interaction existed between the alfalfa cultivars and the rhizobial strains tested, symbiotic effects were influenced by soil conditions obviously, especially the status of soil fertility. The tested alfalfa cultivars ZhongMu No.1 and Golden Empress showed better growth performance inoculated with the strains 73317, 01006, 01055 and 83092, 01006, 01055 respectively.

关 键 词：紫花苜蓿 根瘤菌 接种效果 

分 类 号：S542.9[农业科学—作物学]