RP-HPLC测定淫羊藿不同品种和部位中柔藿苷和淫羊藿苷  

RP-HPLC Determination of Rouhuoside and Icariin in Herba Epimedii of Different Species and Part Used

作　　者：贾敏鸽[1] 孙文基[1] 朱朝德 胥道宝 孙全明 周颖新[1] 

机构地区：[1]西北大学生命科学学院中药系 [2]陕西省安康地区药品检验所,安康725000

出　　处：《药物分析杂志》2005年第1期64-67,共4页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:为了对中药淫羊藿中柔藿苷和淫羊藿苷含量进行系统研究,测定了淫羊藿不同品种和药用部位的柔藿苷和淫羊藿苷含量。方法:采用RP-HPLC法对3个品种多个样品进行测定,以BECKMAN COULTER_(TM)-C_(18)柱(带预柱)为色谱柱,流动相为乙腈-水(25∶75),流速1.0mL·min^(-1),检测波长为270nm。结果:柔藿苷在0.19642-1.9642μg范围内呈良好的线性关系,淫羊藿苷在0.19335-1.9335μg范围内呈良好的线性关系。巫山淫羊藿同一植株体的不同部位中柔藿苷和淫羊藿苷的分布均为叶>根>茎。巫山淫羊藿同一植株的任何部位都是柔藿苷含量高于淫羊藿苷,而淫羊藿和箭叶淫羊藿叶中都是淫羊藿苷含量高于柔藿苷。结论:巫山淫羊藿中以柔藿苷为主要成分,淫羊藿和箭叶淫羊藿中以淫羊藿苷为主要成分。Objective: In order to determinate the content of rouhuoside and icariin in Chinese traditional medicine-Herba Epimedii, several samples from different species and their different parts were examined. Methods: Nineteen samples from 3 species and their different parts were dealt with by HPLC with BECKMAN COULTER TMC_(18) analytical column. The mobile phase consisted of acetonitrile-water(25: 75). The flow rate was 1. 0mL·min^(-1). The UV detection was set at 270nm. Results: The linear response range from 0. 19642-1. 9642μg of rouhuoside(r=0. 99912, n=10);0. 19335-1. 9335μg of icariin(r=0. 99900, n=10). The content of rouhuoside is higher than that of icariin in Epimedium wushanense, while the content of rouhuoside is lower than that of icariin in Epimedium brevicornum and Epimedium sagittatum. Conclusion: As far as both the contents of rouhuoside and icariin in Epimedium wushanense to be concerned, it was obviously different in different parts used(leave, root and stem)of one whole plant. Rouhuoside was the main component in Epimedium wushanense, and icariin was the main component in Epimedium brevicornum and Epimedium sagittatum.

关 键 词：淫羊藿苷 含量 RP-HPLC测定 部位 箭叶淫羊藿 同品种 RP-HPLC法 植株 主要成分 C18柱 

分 类 号：R284[医药卫生—中药学] R286.0[医药卫生—中医学]