柳氮磺胺嘧啶对大鼠移植心脏心肌白细胞介素-1β和细胞间黏附分子-1表达的影响  

Effect of sulfasalazine on expression of inteleukin-1 beta and intercellular adhesion molecule-1 in acute rejection of rat heart transplantation

作　　者：李寒[1] 郅兴义[1] 

机构地区：[1]郑州大学第一附属医院胸心外科,郑州450052

出　　处：《郑州大学学报（医学版）》2005年第1期105-107,共3页Journal of Zhengzhou University(Medical Sciences)

摘　　要：　　　目的: 探讨柳氮磺胺嘧啶 (SSA)对移植心脏的保护作用。方法:建立大鼠异位心脏移植模型,分为 3组:Wistar到Wistar大鼠为对照组, SD到Wistar大鼠为同种移植组, 同种移植SSA治疗组。于移植术后第 3d、5d、7d检测移植心肌组织IL- 1β、ICAM -1,并观察排斥反应发生情况。结果:对照组心肌组织术后第 3d, 5d, 7dIL 1β分别为(124. 89±3. 29)ng/g, (126. 96±2. 73)ng/g, (124. 25±2. 87 )ng/g;ICAM 1分别为 ( 144. 40±22. 11 ),(145. 51±1. 73), (147. 61±2. 05);表达均微弱。同种移植组各时间点IL 1β分别为(154. 48±2. 42)ng/g, (336. 42±3. 24), (336. 72±2. 75)ng/g;ICAM 1分别为(177. 38±2. 16), (196. 16±2. 39), (196. 43±2. 22),与对照组相比均增高(P<0. 05 );SSA治疗组IL 1β分别为 (135. 13±2. 28)ng/g, (145. 50±2. 08 )ng/g, ( 153. 28±1. 73 )ng/g;ICAM 1分别为(153. 95±1. 66), (154. 97±1. 98), (145. 04±2. 36);表达均较同种移植组降低 (P<0. 01 )。对照组心肌组织结构基本正常,同种移植组表现出不同程度的淋巴细胞浸润、心肌间质水肿和组织坏死; SSA治疗组心肌损伤程度降低。结论:大鼠心脏移植急性排斥反应中IL- 1β、ICAM -1的升高,对移植心脏起到保护作用。Aim: To investigate the protective effects of sulfasalazine(SSA) on transplanted rat heart. Methods:A rat heterotopic cardiac transplant model with SD donors and Wistar recipients was used.Three groups in total: control group of Wistar to Wistar rat heart transplantation, group of SD to Wistar rat heart transplantation, group of SD to Wistar rat heart transplantation and treated with SSA. At 3 d,5 d,and 7 d after operation,harvested grafts received immunohistochemical and histopathological examinations to examine the expression of ICAM-1 and acute rejection. Radioimmunoassay and multimedia pathology imaging analysis system were used for quantitative measurement of IL-1β and ICAM-1 expression.Results: Faint expression of IL-1βand ICAM-1 at different time points in control group was found; in heterotopic transplantation group, IL-1β and ICAM-1 contents significantly elevated(P<0.05); after treatment with SSA, IL-1β and ICAM-1 contents significantly decreased(P<0.01). In control group the cardiac tissue was nearly normal;in heterotopic transplantation group,infiltrated lymphocytes, myocardium stromatic edema, and tissue necrosis could be observed the injury of grafts was significantly relieved by SSA. Conclusion: SSA can suppress the excessive expression of IL-1β and ICAM-1 during the acute graft rejection and protect the transplanted rat hearts.

关 键 词：大鼠 心脏移植 急性排斥反应 白细胞介素-1 细胞间黏附分子-1 柳氮磺胺嘧啶 

分 类 号：R654.2[医药卫生—外科学]