CB法微核检测技术应用于诱导基因组不稳定性的研究  

Genomic instability induced by γ-ray irradiation analyzed by cytokinesis-block MN assay in CHL cell line

作　　者：沈波[1] 沈冰[2] 

机构地区：[1]吉林大学公共卫生学院卫生部放射生物重点实验室,长春130021 [2]中国辐射防护研究院科技信息中心,太原030006

出　　处：《辐射研究与辐射工艺学报》2005年第1期45-48,共4页Journal of Radiation Research and Radiation Processing

摘　　要：对γ 射线诱导中国仓鼠成纤维(CHL)细胞基因组不稳定性进行了研究。利用 CB 法微核检测技术对一次照射、二次照射的 CHL 细胞进行微核测定,观察 DNA 损伤程度的变化情况。结果显示,微核发生率与细胞受照剂量呈相关关系,并随培养时间延长呈下降趋势。细胞再次受照后,其微核发生率与一次受照剂量呈相关关系。表明微核发生率与染色体损伤程度存在剂量相关性,微核检测技术可以用于生物效应表现为染色体损伤的辐射诱导基因组不稳定性的研究。To analyze the genomic instability induced by γ-rays, cytokinesis-block MN assay was employed to investigate the gene damage of cells and their progeny cells exposed to radiation. The micronucleus frequency were related to the irradiation dose and decreased as the culture time lengthened. There was a correlation between the micronucleus frequency and the initial dose after the second irradiation. The micronucleus frequency may be causally related to radiation-induced genomic instability which is expressed as chromosomal damage leading to micronuclei.

关 键 词：基因组不稳定性 CB法微核检测技术 

分 类 号：R811.5[医药卫生—放射医学]