肿瘤坏死因子-α诱导大鼠脂肪细胞凋亡  

Rat Adipocyte Apoptosis Induced by Tumor Necrosis Factor-α

作　　者：林亚秋[1] 李瑞文[1] 孙超[1] 陈国柱[1] 杨永青[1] 孟德连[1] 杨公社[1] 

机构地区：[1]西北农林科技大学动物脂肪沉积与肌肉发育实验室,杨凌712100

出　　处：《农业生物技术学报》2004年第6期644-647,共4页Journal of Agricultural Biotechnology

基　　金：国家教委博士点基金(No.1998071232)

摘　　要：采用光学显微镜、DNA琼脂糖凝胶电泳和流式细胞术3种技术,检测了不同浓度肿瘤坏死因子α(TNF-α)对大鼠脂肪细胞凋亡的影响。结果表明,①TNF-α诱导脂肪细胞凋亡的形态学变化与浓度呈线性相关,在5～20 ng/mL范围内,浓度越大,凋亡特征越明显。②5 ng/mLTNF-α处理有明显的形态学变化,但电泳结果未见梯状条带,说明细胞凋亡的形态学变化早于细胞凋亡的生化特征性改变。③TNF-α诱导脂肪细胞凋亡在5～20 ng /mL浓度范围内存在剂量.效应关系.5.10,15和20ng/mL TNF-α处理组与对照组相比差异极显著(P<0.01),lO ng/mL与15和20 ng/mL处理组之间差异不显著(P>0.05),以10 ng/mL,为最佳诱导浓度。The effects of tumor necrosis factor-α(TNF-α) at different concentration levels on rat adipocyte apoptosis were detect- ed by optical microscopy, agarose gel electrophoresis and flow cytometry methods. The morphologic changes of rat adipocyte apopto- sis induced by TNF-α were correlated linearly with the concentrations of TNF-α within a range from 5 to 20 ng/mL, and the higher concentration of TNF-α could induce more obvious apoptosis. The morphologic changes of rat adipocytes treated by 5 ng/mL TNF-α were clearly shown, but their DNA ladders didn't appear by the DNA electrophoresis analysis, indicating that the morphologic changes occurred earlier than the biochemical changes. TNF-α induced the rat adipocyte apoptosis in a dose-dependent manner within a concentration range from 5 ng/mL to 20 ng/mL. Compared with the control, the apoptotic effect induced respectively by 5, 10, 15 and 20 ng/mL TNF-α was significantly difference (P<0.01), but the effect among 10, 15 and 20 ng/mL TNF-α treatments was not dif- ference (P>0.05). Thus the optimum concentration of TNF-α inducing apoptosis was determined at the level of 10 ng/mL.

关 键 词：凋亡 脂肪细胞 诱导 形态学变化 大鼠 肿瘤坏死因子-Α 处理 浓度 生化特征 剂量 

分 类 号：S965[农业科学—水产养殖] R735[农业科学—水产科学]