转基因小鼠生产过程的优化  

Optimization on Process of Transgenic Mice Production

作　　者：郑敏 王莉莉[2] 王美丽 牛慧玲 戴蕴平[2] 

机构地区：[1]北京基因达生物科技有限公司,北京100094 [2]中国农业大学农业生物技术国家重点实验室,北京100094

出　　处：《农业生物技术学报》2004年第6期672-675,共4页Journal of Agricultural Biotechnology

摘　　要：以昆明小鼠作为制备转基因小鼠的供、受体,优化了转基因小鼠生产过程中的显微注射和移植过程,调整了对大小不同的DNA的注射浓度和受体鼠的选择标准。结果使转基因效率从10％上升到20％以上;并得出DNA的大小与注射浓度的最适结合点为大于10 kb的DNA,浓度应控制在2～3μg/mL。The microinjection and implantation conditions were optimized by Kunmin mice as donor and receipt in the pro- duction of transgenic mice. Simultaneously the DNA concentrations in different size and standard of receipt selecting were redressed. Eventually the positive rates of transgenic mice were improved from 10% to 20%; the optimum suitability of DNA concentration was 2～3 μg/mL when size of DNA fragment exceed 10 kb.

关 键 词：转基因小鼠 生产过程 优化 显微注射 胚胎移植 转基因效率 实验动物 

分 类 号：S865.1[农业科学—野生动物驯养] Q785[农业科学—畜牧兽医]