酸胁迫对虎纹蛙红细胞及血红蛋白的影响  

Influence of Erythrocyte (RBC) and Hemoglobin (Hb) in Tiger Frog Rana tigerina rugulosa Wiegmann Under of Acid Stress

作　　者：邵晨[1] 施时迪[2] 谢建芳[1] 

机构地区：[1]浙江师范大学化学与生命科学学院生物科学系,浙江金华321004 [2]浙江台州学院生命科学与医药化工学院,浙江临海317000

出　　处：《农业环境科学学报》2005年第1期39-42,共4页Journal of Agro-Environment Science

基　　金：浙江省自然科学基金(M303128)

摘　　要：采用室内水族箱培养方法,研究了不同酸梯度对虎纹蛙(RanatigerinarugulosaWiegmann)红细胞和血红蛋白的影响。结果表明,在pH3.5～pH5.0之间,酸胁迫对虎纹蛙的红细胞数量及血红蛋白含量具有较明显的剂量效应,其中pH3.5条件下对虎纹蛙红细胞和血红蛋白产生了较强的毒害作用,并且随着时间的推移对红细胞及血红蛋白的影响呈现逐渐加重的趋势。随着pH值的上升,其红细胞数量和血红蛋白含量也逐渐增大。pH值≥pH5.5可能是虎纹蛙较为适宜的水体环境。试验表明,酸胁迫可对虎纹蛙造成较为严重的危害,可将虎纹蛙红细胞数量和血红蛋白含量的变化作为检测水体酸污染的指标和手段。The influence of erythrocyte (RBC) and hemoglobin (Hb) in different consistencies to wild Tiger Frog Rana tigerina rugulosa Wiegmann) under acid stress was researched in this study. To do the test, several tiger frogs were captured from the Mountain Nanshan in Jinhua and the Mountain Kuocangshan, respectively, in Taizhou, Zhejiang Province, China. For the experiment, five testing groups were exposed under different acid concentrations from pH3.5 to 5.5(tap water, adjusting the pH with 0.05 mol·L-1 H2SO4 and 0.1 mol·L-1NaOH) and one control ( water only, pH≥6.5) were utilized in laboratory. The blood samples of two experimental animals from every group were obtained after 24, 48, 72, 96, 120, 144 and 168 h, separately, and the numbers of RBC and the contents of Hb in the tested animals were tested. The results indicate that the influence of acid stress towards the number of RBC and the contents of Hb exhibited harmful effects between pH3.5 and pH5.0, and especially much poisonous effect at pH3.5. The effects of acid stress to RBC and Hb in the frogs became more and more significant as the exposure period prolong. The number of RBC and the contents of Hb gradually augmented as pH raised, suggesting that pH≥pH 5.5 be the suitable environment to Rana tigerina rugulosa Wiegmann. From the results of the test above,the acid stress was more harmful to tiger frog Rana tigerina rugulosa Wiegmann.In the mean time, variations of the numbers of RBC and the contents of Hb of the animal may be regarded as an index and a means of testing acid pollution in water. The tiger frog is beneficial to agriculture, so it's very important to prevent harming this animal from acid stress in environmental protection.

关 键 词：酸胁迫 红细胞 血红蛋白 虎纹蛙 

分 类 号：X503.225[环境科学与工程—环境工程]