前脑啡肽原和热休克蛋白参与宿主对结核分枝杆菌感染的应答  

Preproenkephalin and Heat Shock Protein Involving in the Response to Mycobacterium tuberculosis Infection: Revealed by a Combination of Transcriptomic and Proteomic Approach

作　　者：徐霖[1,2] 乐军 刘丽蓉[2] 梁莉[3] 王洪海[2] 谢建平[2,4] 

机构地区：[1]贵阳师范高等专科学校化学系,贵州贵阳550008 [2]复旦大学生命科学学院遗传工程国家重点实验室 [3]上海市肺科医院,上海200433 [4]西南师范大学生命科学学院现代生物医药研究所

出　　处：《西南师范大学学报（自然科学版）》2005年第1期126-130,共5页Journal of Southwest China Normal University(Natural Science Edition)

基　　金：国家重大基础研究973资助项目(2002CB512804);国家自然科学基金资助项目(3010007).

摘　　要：利用双向电泳比较了由临床分离的结核分枝杆菌感染离体巨噬细胞 U937 前后的全细胞蛋白组表达差异,肽指纹鉴定和生物信息学分析2个表达明显上调的斑点, 确定它们分别为热休克蛋白 HSP105β和前脑啡肽原. 比较蛋白质组学和转录组学研究发现, 热休克蛋白表达提高可能源于转录增加. 提出了从神经免疫学角度研究结核分枝杆菌致病和宿主免疫机理的新思路, 为解释吸毒人群中结核病高发病率提供了基础.Two-dimensional electrophoresis was used to compare the proteome of macrophage cell line U937 before and after Mycobacterium tuberculosis clinical isolates infection. Two spots upregulated markedly after infection was subjected to peptide fingerprint and bioinformatics analysis. The two spots were identified as heat shock protein 105 beta and preproenkephalin respectively. The upregulation of heat shock protein 105 beta was possibly the increasing of transcription revealed by a combination of proteomic and transcriptomic approach. This was the first report on the upregulation of host cell endogenous preproenkephalin and heat shock protein 105 beta by M.tuberculosis clinical isolates infection. This study is another clue to further investigation of Mycobacterium tuberculosis pathogenesis and host immune strategy from neuroimmunology. It helps to explain the high incidence of tuberculosis among drug abusers.

关 键 词：前脑啡肽原 热休克蛋白 巨噬细胞U937 结核分枝杆菌 蛋白质组 

分 类 号：Q93[生物学—微生物学]