高效液相色谱-串联质谱法鉴定中成药中的格列本脲  

Identification of Glibenclamide in Traditional Chinese Medicines by High-performance Liquid Chromatography/Tandem Mass Spectrometry

作　　者：姚骏骅[1] 周丽华[1] 张东娣[1] 查庆民[1] 

机构地区：[1]中山大学测试中心,广东广州510275

出　　处：《中山大学学报（自然科学版）》2005年第1期53-56,60,共5页Acta Scientiarum Naturalium Universitatis Sunyatseni

基　　金：广东省自然科学基金资助项目(990209)

摘　　要：建立了一种简便、快速、灵敏、准确、可靠地鉴定中成药制剂中是否添加格列本脲的高效液相色谱-串联质谱(HPLC＿MS/MS)分析方法。HPLC色谱柱为ThermoHypersilBDSC8柱(4 6mm×150mm,5μm);流动相为甲醇/乙腈/水(含φ=0 1%HAc)的体积比为55∶15∶30;流速为0 5mL·min-1。HPLC＿MS与HPLC＿MS/MS以离子阱为检测器,采用正、负2种离子检测模式进行检测。运用此方法成功地确证了3种用于治疗糖尿病的中西复方药中均含有格列本脲。A method of high_performance liquid chromatography/tandem mass spectrometry (HPLC_MS/MS) was developed for the identification of glibenclamide added in Chinese traditional medicines. HPLC separation was carried out on a BDS C8 column (4.6 mm×150 mm, 5 μm ) using an isocratic mobile phase (CH_3OH/CH_3CN/0.1% HAc 55∶15∶30 ) in less than 15 min. HPLC_MS and HPLC_MS/MS were performed on an ion trap instrument in both positive and negative ionization mode. This method is simple, rapid, sensitive and reliable, and has been successfully applied to identify glibemclamide in three antidiabetic Chinese traditional medicines.

关 键 词：高效液相色谱-质谱/质谱 格列本脲 中成药 

分 类 号：O657.63[理学—分析化学]