中国特有极度濒危植物猪血木的保护遗传学研究  

Genetic Differentiation and Conservation of 14 Surviving Individuals of Euryodendron excelsum Endemic to China

作　　者：王艇[1] 苏应娟[1] 叶华谷[2] 欧阳蒲月[1] 江宇[1] 孙宇飞[1] 陈国培[1] 邓锋[1] 张宏达[1] 

机构地区：[1]中山大学生命科学学院,广东广州510275 [2]中国科学院华南植物园,广东广州510650

出　　处：《中山大学学报（自然科学版）》2005年第1期68-72,共5页Acta Scientiarum Naturalium Universitatis Sunyatseni

基　　金：国家自然科学基金资助项目(30271094);留学回国人员科研启动基金(外教司留[2003]406号)

摘　　要：应用RAPD标记分析了广东阳春八甲镇猪血木EuryodendronexcelsumH T Chang种群全部14个个体的遗传变异和进化关系。23个引物共检测到156个位点,其中多态位点95个,多态位点比率60 90%。根据所得数据求出的观察等位基因数为1 6090、有效等位基因数为1 3471、Nei基因多样性为0 1993、Shannon多样性指数为0 1534。猪血木还保留有比较丰富的遗传多样性。对Jaccard相似性系数用UPGMA法进行聚类分析表明,猪血木个体明显地分为2个亚群,其中2号个体同其他成员的遗传关系疏远。RAPD谱带表型的主成分分析(PCA)支持聚类分析结果。根据这些结果讨论了种群的管理和保护策略。RAPD markers were used to assess the genetic variations and the evolutionary relationships among all 14 individuals of a critically endangered Euryodendron excelsum (Theaceae) population remaining at Ba Jia Zhen, Yangchun, Guang dong, China. Twenty_three random primers detected 156 sites, out of which 95 (60.26%) were polymorphic loci. Number of observed alleles was 1.6090, number of effective alleles was 1.3471, Nei's gene diversity was 0.1993, and Shannon index was 0.1534. Relatively high level of genetic variation was identified in E. excelsum. UPGMA tree established from Jaccard similarity coefficients exhibited that 14 individuals were clustered into two subgroups, showing that the number 2 plant was genetically distant from the rest of plants. The UPGMA clustering was also lent support by a principle components analysis (PCA) of RAPD phenotypic data. Management and conservation strategy of E. excelsum was proposed based on our results.

关 键 词：猪血木Euryodendron excelsum H.T.Chang 种群变异 RAPD标记 保护策略 

分 类 号：Q943[生物学—植物学] Q347