卤虫HOX基因PCR扩增条件的优化  

Optimization of PCR Conditions for HOX gene in Artemia

作　　者：陈志娟[1] 侯林[1] 林盛国[1] 徐明玉[1] 王璐[1] 

机构地区：[1]辽宁师范大学生命科学学院,辽宁大连116029

出　　处：《水产科学》2005年第2期12-14,共3页Fisheries Science

基　　金：国家自然科学基金资助项目(30271035)

摘　　要：在研究卤虫的HOX基因abdA基因的分子进化中,为了可靠地扩增出两性生殖卤虫的abdA基因,从模板浓度、引物浓度和Taq酶浓度三个方面对PCR反应体系进行了优化,同时设定了4种退火温度,进行对比试验。扩增的结果经过琼脂糖电泳检测,发现在25μl的反应体系中,引物浓度为5pmol、Taq酶浓度在1 25U,模板为母液浓度不稀释时效果较好,同时本研究还得出为了扩增abdA基因,退火温度在55℃时最佳。During the study of molecular evolution of HOX gene in Artemia.We optimized PCR conditions in order to amplify the abda gene exactly from genome DNA in bisexual Artemia. in three aspects: the density of template, the density of primer, the density of Taq. At the same time we designed 4 melting temperatures, and conductes a series of comparative experiments . The results showed that the profiles of PCR product s could be improved obviously by the density of template at 60ng,the density of primer at 5pmol and Taq 1.25u with a meltting temperature of 55 ℃.

关 键 词：HOX-abdA基因 PCR扩增 条件优化 

分 类 号：Q959.223[生物学—动物学]