瘦肉型猪(PIC344)精液酸性磷酸酶功能基团的化学修饰  

Effect of chemical reagents on acide phosphatase from sperm of PIC pigs(PIC344)

作　　者：姜骅[1] 张勇侠[2] 李佳[1] 国锦琳[1] 肖舸[1] 刘克武[1] 

机构地区：[1]四川大学生命科学院,四川成都610064 [2]四川抗菌素工业研究所,四川成都610051

出　　处：《化学研究与应用》2005年第1期36-38,共3页Chemical Research and Application

基　　金：四川省成都市大熊猫繁殖基地基金资助项目

摘　　要：不同浓度化学修饰剂二巯基苏糖醇(DTT)、对氯汞苯甲酸(pCMB)、N 溴代琥珀酰亚胺(NBS)、苯甲基磺酰氟(PMSF)、溴代乙酸(BrAc)的修饰能抑制瘦肉型猪(PIC344)精液酸性磷酸酶(ACPase,E C 3 1 3 2)的活力。同一浓度不同种类的修饰剂修饰酶活力降低程度不同。化学修饰剂修饰ACPase不同时间后,酶活力均有不同程度的降低,其中PMSF作用60min后酶活力仅存18%,而BrAc的修饰对ACPase的活力无明显影响。Acid Phosphatase(ACPase,E.C.3.1.3.2) was modified by NBS,PMSF,pCMB,DTT and AcBr.The effects of differert concentration and time on the ACPase catalytic activities were determined.The reactions of ACPase from PCI344 with NBS,PMSF,pCMB and DTT resulted in a strong inhibition of enzyme activities.The activities of ACPase decreased steadily with the increase of modification reagents concentration and time.Histidine residues modified by BrAc did not change the activity of the enzyme,while the activity only remained 18%after PMSF modified ACPase for 60min.

关 键 词：瘦肉型猪精液(PIC344) 酸性磷酸酶 化学修饰 

分 类 号：O629.8[理学—有机化学]