柱头场放大样品富集技术测定镍  

Head-column field-amplified sample stacking in capillaryelectrophoresis—an effective on-line sample stacking technique

作　　者：刘彦明[1] 王辉[1] 韩金土[1] 王军华[2] 程介克[2] 

机构地区：[1]信阳师范学院化学化工学院,河南信阳464000 [2]武汉大学化学与分子科学学院,湖北武昌430072

出　　处：《化学研究与应用》2005年第1期52-54,共3页Chemical Research and Application

摘　　要：利用毛细管电泳在线化学发光检测技术,研究了镍离子场放大样品富集过程。结果表明,利用该技术可使灵敏度显著改善,浓缩因子达5 8×104,镍离子检出限为1 2×10-10mol/L。预进一段水柱可使检出限达到7 0×10-11mol L。还考察了进样方式、样品基体浓度、进样时间、进样电压对检测灵敏度的影响。Field-amplified sample stacking of nickel(Ⅱ)was investigated using capillary electrophoresis with chemiluminescence detection for the first time.The sensitivity of nickel(Ⅱ)was improved markedly by the field-amplified sample stacking technique and the detection limit reached 1.2×10^(-10)mol/L.By injection of a short plug of water before sample introduction,the sensitivity was further improved and the detection limit reached 7.0×10^(-11)mol/L.Parameters affecting the sensitivity,such as modes of injection,concentration of sample matrix,injection time,injection voltage and a short plug of water before sample introduction,were investigated in detail.

关 键 词：毛细管电泳 化学发光检测 镍 场放大样品富集 

分 类 号：O657.8[理学—分析化学]