猪胸膜肺炎放线杆菌、猪多杀性巴氏杆菌、副猪嗜血杆菌复合PCR检测方法的建立  

Development of a Multiplex PCR for Detecting Actinobacillus Pleuropneumoniae, Pasteurella Multocida and Haemophilu Parasuis

作　　者：米丰泉[1] 陈小玲[2] 赵玉军[1] 王翠敏[1] 王凤强[1] 张彦[1] 

机构地区：[1]沈阳农业大学畜牧兽医学院 [2]北京市农林科学院畜牧兽医研究所

出　　处：《饲料工业》2005年第4期38-40,共3页Feed Industry

摘　　要：在已经建立的猪胸膜肺炎放线杆菌(APP)、猪多杀性巴氏杆菌(PM)、副猪嗜血杆菌(HPS)的单项PCR诊断方法的基础上,通过对扩增条件的筛选,成功地建立了APP、PM、HPS复合PCR诊断方法,并应用于临床。利用一次PCR反应,即可同时扩增APP的342bp、PM的457bp和HPS的821bp的特异性片段。该方法的建立对临床上进行这3种疾病的鉴别诊断和混合感染的检测都具有重要意义。TA multiplex PCR detecting App, Pm and Hps has been developed based on published single PCRs for these pathogens. The PCR reagents, annealing temperature and the PCR procedure were optimized. A 342bp fragment of App, a 457bp of Pm and a 821bp of Hps can be simultaneously amplified in one reaction. The assay is a potential tool for differentiation of the 3 pathogens and diagnosis of mixed infection by these bacteria.

关 键 词：猪胸膜肺炎放线杆菌 猪多杀性巴氏杆菌 副猪嗜血杆菌 复合PCR 

分 类 号：S858.28[农业科学—临床兽医学]