利用高效检测菌株对中慢生根瘤菌及红壤中自体诱导物的检测  

DETECTION OFAUTOINDUCER IN MESORHIZOBIUM AND RED SOIL

作　　者：钟增涛[1] 周晶[1] 李路[2] 高轶静[1] 朱军[1] 

机构地区：[1]南京农业大学农业环境微生物工程重点开放实验室,南京210095 [2]南京师范大学生命科学学院生物技术系,南京210097

出　　处：《土壤》2005年第1期61-64,共4页Soils

基　　金：国家杰出青年基金(30325004);霍英东基金(91023);自然科学基金项目(30200036)资助。

摘　　要：利用高效的自体诱导物(AI)检测菌株 KYC55 检测中慢生根瘤菌属不同种菌株产生的 AI,发现AI 在中慢生型根瘤菌中广泛存在,AI 在酸性条件下比较稳定。选用酸性土壤(红壤)作为材料,乙酸乙酯抽提并检测其 AI,发现生境条件、采样时间及保存条件都对 AI 的稳定性有影响。Autoinducers from different strains of Mesorhizobium were detected with Strain KYC55(pJZ372) (pJZ384) (pJZ410). The results show that autoinducers widely exist in Mesorhizobium, and pH of the medium affects activities of the autoinducers seriously. Acidic condition is suitable for stability of the autoinducers. So red soil was chosen for extraction of autoinducers with ethyl acetate. It was found that the autoinducer activities varied with the ecological environments, sampling times and preservation conditions.

关 键 词：群体感应 中慢生根瘤菌 红壤 自体诱导物 

分 类 号：S154[农业科学—土壤学] S154.381[农业科学—农业基础科学]