人骨髓间充质干细胞的分离培养及向成骨细胞定向分化的实验研究  

Experimental Research on mMSC_S Isolation, Culture and its Differentiation Towards Osteoblasts

作　　者：康非吾[1] 吴正华[2] 黄欣[3] 唐休发[3] 温玉明[3] 

机构地区：[1]同济大学口腔医学院口腔颌面外科,上海200072 [2]山东大学齐鲁医院口腔颌面外科,济南250012 [3]四川大学华西口腔医院口腔颌面外科,成都610041

出　　处：《口腔颌面外科杂志》2004年第4期307-310,共4页Journal of Oral and Maxillofacial Surgery

基　　金：同济大学科技发展基金项目(20041876)

摘　　要：目的:建立人骨髓间充质干细胞(hMSCS)体外分离、培养体系,并将其向成骨细胞定向诱导分化。方法: 采用梯度离心法从人骨髓血中提取骨髓MSC,经体外培养扩增,观察其生物学特性。结果:在体外培养条件下,骨 髓MSC具有较强的增殖能力,并能被诱导向成骨细胞分化。结论:建立了骨髓MSC体外分离、培养的体系,探讨 了骨髓MSC的生物学特性,为进一步研究MSC作为骨组织工程的种子细胞打下了基础。Objective: To establish the method of mesenchymal stem cells (MSC) isolation and culture in vitro, and induce MSC differentiation towards osteoblasts. Methods: Human marrow was obtained by puncture and gradient centrifuga-tion performed to isolate cell contains MSCs, and its biological feature was observed by cell culture. Results: hMSCs showed active proliferative capacity in vitro, and it can be induce to osteoblasts by using osteogenic induce culture. Conclusion : The condition for isolation and culture of hMSCs were established successfully, and some biological feature were observed. Which create a base for the further research using hMSCs as seed cells for bone tissue engineering.

关 键 词：骨髓间充质干细胞 细胞培养 成骨细胞 骨组织工程 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]