草鱼出血病病毒人工感染稀有(鱼句)鲫出血病鱼主要器官组织的超薄切片观察  

OBSERVATIONS ON THE ULTRA-THIN SECTIONS OF THE MAIN ORGANS AND TISSUES OF HEMORRHAGIC GOBIOCYPRIS RARUS ARTIFICIALLY INFECTED BY GRASS CARP HEMORRHAGIC VIRUS (GCHV)

作　　者：王铁辉[1] 陈宏溪[1] 刘沛霖[1] 刘汉勤[1] 郭文[1] 易泳兰[1] 

机构地区：[1]中国科学院水生生物研究所,武汉430072

出　　处：《水生生物学报》1993年第4期343-346,共4页Acta Hydrobiologica Sinica

基　　金：淡水生态与生物技术国家重点实验室课题

摘　　要：用电子显微镜观察了稀有(鱼句)鲫出血病(Hemorrhage of Gobiocypris rarus)鱼的病理切片及对照鱼的超薄切片。在对照鱼的鳃、肠道、肾脏、脾脏、肌肉和肝脏中没有见到任何病毒颗粒。在病鱼鳃血管内皮细胞质中观察到聚集的直径70nm左右的GCHV颗粒,说明鳃是GCHV侵袭的主要器官之一,并讨论了鳃对GCHV敏感在GCHV传播中的意义。还在病鱼肠道,肾脏中观察到成片的病毒颗粒,它们也是GCHV侵袭的主要组织。在病鱼脾脏的电子密度低的细胞质中观察到散在的病毒颗粒。很少见到病毒聚集体,脾脏中的病毒可能是脾脏的吞噬细胞吞噬而来。作者认为在病鱼组织中观察到的大小不同的病毒颗粒是处于不同成熟阶段的GCHV。Hemorrhage of Gobiocypris rarus was induced by artificial infection of Grass carp hemor rhagic virus (GCHV). The ultra-thin sections of the tissues of the control and diseased fish were studied under electron microscope. No virions were discovered in the tissues of the control. The discovery of the aggregated GCHV particles in the endothelial cell cytoplasm of gill blood vessel indicated that the gill is one of the main organs infected by GCHV and this organ may take an important part in the spread and infection of GCHV. The aggregates of GCHV particles present in the cytoplasm of intestine and kidney cells suggested that the intestine and kidney are the other main organs of GCHV infection. In the spleen of the diseased fish, single virions were discovered in the cytoplasm of the cells with low electron density, and virion aggregates were rarely observed. These virions may come from the phagocytosis of phagocyte. ☆We hold the view that the two kinds of viral particles, 40nm and 70nm in diameter respectively, represent different developmental stages of the GCHV.

关 键 词：稀有Ju鲫 草鱼 出血病 病毒 

分 类 号：S941.411[农业科学—水产养殖]