HIV-2gag-gp105嵌合基因DNA疫苗对小鼠的免疫原性研究  

Humoral and Cellular Immunogenecity of DNA Vaccine of HIV-2 Chimeric Gene gag-gp105 in BALB/c

作　　者：李子健[1] 金宁一[2] 张立树[2] 江文正[2] 张洪勇[2] 

机构地区：[1]北京大学第三医院血管医学研究所,北京100083 [2]军事医学科学院军事兽医研究所全军基因工程重点实验室,吉林长春130062

出　　处：《中国病毒学》2004年第6期559-562,共4页Virologica Sinica

基　　金：国家"863"基金资助(2001AA215031);吉林省科技发展计划项目(20030550-1)

摘　　要：利用真核表达载体 pVAX1 构建 HIV-2 gag-gp105 嵌合基因的重组质粒 pVAX1gag-gp105,将其转入 BHK21细胞中,利用间接免疫荧光方法检测其表达情况。进一步分别将核酸疫苗质粒 pVAX1gag-gp105、对照组质粒pVAX1 和 PBS 溶液经肌肉注射免疫 BALB/c 小鼠,检测免疫小鼠脾 CD4+、CD8+T 细胞亚群的数量,脾特异性 CTL杀伤活性和血清抗体滴度。结果显示,重组核酸疫苗质粒 pVAX1gag-gp105 疫组小鼠脾 CD4+、CD8+T 细胞亚群的数值均比对照组高( P<0.01),脾特异性 CTL 杀伤活性与对照组相比差异极显著(P<0.01),血清抗体滴度显著高于对照组(P<0.01) 。以上结果表明,HIV-2 gag-gp105 嵌合基因 DNA 疫苗对 BALB/c 小鼠具有良好的体液和细胞免疫原性。The recombinant expression plasmid of pVAX1gag-gp105 was constructed and transfected into BHK21 cells by lipofectamine, the expressed product was detected by indirect immunofluore scence. After the mice were immunized with pVAX1gag-gp105 and control plasmid, the number of CD4+and CD8+ subgroups of spleen T lymphocyte, the specific killing activities of spleen CTL and the titers of serum antibodies of the immunized mice were detected. Compared with vaccinated control groups, pVAX1gag-gp105 vaccinated group had significant differences in the titers of the serum antibodies (P<0.01), the numbers of spleen CD4+ and CD8+ T cells (P<0.05) and the specific killing activities of spleen CTL (P<0.01). These results manifested that the DNA vaccine based on HIV-2 chimeric gene gag-gp105 possessed good humoral and cellular immunogenecity in BALB/c mice.

关 键 词：HIV-2 gag-gp105 DNA疫苗 

分 类 号：R392[医药卫生—免疫学]