针刺对糖尿病合并脑缺血大鼠海马细胞色素氧化酶的影响  

Effect of Acupuncture on the Activity of Hippocampal Cytochrome Oxidase in Rats with Diabetes plus Cerebral Ischemia

作　　者：景向红[1] 蔡虹[1] 陈淑莉[1] 逯波[1] 蒋瑾[1] 晋志高[1] 

机构地区：[1]中国中医研究院针灸研究所,北京100700

出　　处：《针刺研究》2004年第4期257-260,265,共5页Acupuncture Research

摘　　要：目的 :探讨细胞色素氧化酶 (COX)在糖尿病合并脑缺血发病机制中的作用及针刺对其的影响。方法 :采用腹腔注射链脲佐菌素 (Streptozotocin ,STZ)建立糖尿病动物模型 ,注射后 3d用颈总动脉阻断法建立脑缺血模型。注射STZ 7d后测定空腹血糖 ,高于 1 5.5mmol/L定为糖尿病模型 ,术后 1个月经行为学测定确定糖尿病脑病模型。动物分为正常对照组 (n =5)、糖尿病 +假手术组 (n =5)、糖尿病 +脑缺血组 (n =6)、糖尿病 +脑缺血 +针刺组 (n =6) ,采用组织化学方法观察大鼠海马CA1区和CA3区COX活性反应物的分布 ,用图像分析仪对其光密度进行测定。结果 :糖尿病脑缺血组的大鼠海马CA1和CA3区的COX光密度明显低于正常 (P <0 .0 0 1 ) ,而针刺组高于糖尿病脑缺血组 (P <0 .0 0 1 )。结论 :糖尿病脑缺血可导致大鼠脑内海马CA1和CA3区的COX活性降低 ,针刺可以提高这两个区域的COX活性 ,从而提高细胞代谢的水平。Objective: To explore the role of cytochrome oxida se (COX) in the occurrence o f diabetes mellitus (DM) with concomitant cerebral ischemia and observe the effe ct of acupuncture on COX activity in the hippocampal tissues. Methods: A total o f 22 female Wistar rats, weighing about 150 g, were used in the present study. D M model was established by intraperitoneal injection of streptozotocin ( 60 mg/k g) 4 days before experimental cerebral ischemia. Cerebral ischemia (CI) model was established by using two-vessel (bilateral common carotid arteries) occlusion an d reperfusion method. The occlusion and reperfusion were repeated twice. Bl ood sugar level was detected 7 days after STZ-administration. The animals with th e blood glucose concentration being more than 15.5 mmol/L were selected as diabet ic rats. At the same time, acupuncture of “Baihui”(GV 20), “Sanyinjiao”(SP 6), etc. was given once every other day, 20 sessions altogether. The animals were divided into normal control group (n=5), DM+sham-operation gro up (n=5), DM+CI gr oup (n=6) and DM+CI +acupuncture group (n=6). Af ter treatment the rats were kill ed for collecting brain samples. Cytochemical staining method was used to detect the activity of COX in CA1 and CA3 areas of the hippocampus. COX-positive rea ct ion (30 areas/section) was analyzed with MIS 2000 image analyzer. Resul ts: COX-re action positive neurons and buttons were found in both CA1 and CA3 areas of the hippocampus. The optical density (OD) values of COX-reaction products in DM+CI group (CA1: 2 66 .2±15.6,CA3: 386.1±27.6) were markedly lower than those in normal co ntrol group (CA1: 347.9±66.2,CA3: 462.6±43.8; P<0.001). C omparison between DM +CI+acupuncture group and DM+CI group showed that the OD values of the former gr oup were significantly higher than those of the later group (CA1:336.3±40.8 vs 266.2±15.6,P<0.001; CA3:447.1±36.8 vs 386.1± 27.6, P<0.001). Conclusion: In DM and DM+CI rats, COX activity in the hippocampus lowered or significantly l

关 键 词：糖尿病 脑缺血 针刺 细胞色素氧化酶 海马 

分 类 号：R246.1[医药卫生—针灸推拿学]