利用改进的差异显示技术分离烟草中受乙烯利诱导的新基因(英文)  

Cloning and Characterization of Novel Tobacco Genes Induced by Ethephon Using Improved Differential Display Method

作　　者：余涛[1] 易平[1] 支立峰[1] 李阳生[1] 朱英国[1] 

机构地区：[1]武汉大学生命科学学院植物发育生物学教育部重点实验室,湖北武汉430072

出　　处：《作物学报》2005年第1期24-28,共5页Acta Agronomica Sinica

基　　金：国家 973基金项目 (2 0 0 1CB10 88)资助

摘　　要：利用改进的差异显示技术从烟草中分离受乙烯利调控的新基因。在分离到的 2 4个差异片段中 ,通过点杂交验证 ,表明其中 17个为阳性。对这些阳性片段的序列分析表明有 4个新基因 (DF6 ,DF8,DF9,DF13)。其中DF8是一个未知基因 ,其他 3个通过GenBank搜索可以找到一些同源的已知基因。RT PCR分析表明 ,DF6、DF8、DF13在对照中也有表达 ,受乙烯利诱导其表达水平上调。而DF9则仅在处理植株中表达。An improved differential display approach was used to identify novel genes induced by the ethylene generator ethephon from tobacco leaves.Total 24 cDNA fragments that show different expression level between ethephon-treated and control plants were isolated.Dot blotting indicated that 17 of them were positive.Sequence analysis revealed that four of them (DF6, DF8, DF9, DF13) represented novel genes.One of them (DF8) was an unknown gene, the other three were new cDNA fragments with homology with some known genes.RT-PCR analysis of the four novel cDNA clones revealed that three of them (DF6, DF8, DF13) were expressed constitutively in untreated control plants, and had improved mRNA transcript level in ethephon treated tobacco plants.While DF9 mRNA occurred only in the treated plants.

关 键 词：DDRT-PCR 乙烯 烟草 

分 类 号：S572[农业科学—烟草工业]