磁分离酶联免疫法检测IL-4方法的建立  

Methodological Study on Magnetic Affinity Immunoassay for IL-4

作　　者：王东琳[1] 朱勇[1] 李琦[1] 刘振世[2] 谢晶[2] 欧阳为明[1] 金伯泉[1] 

机构地区：[1]第四军医大学免疫学教研室,陕西西安710032 [2]北京倍爱康生物技术股份有限公司,北京100070

出　　处：《标记免疫分析与临床》2004年第4期225-227,共3页Labeled Immunoassays and Clinical Medicine

基　　金：全军医药卫生科研基金课题 (NO .0 12 0 80 )

摘　　要：建立磁分离酶联免疫法定量检测人白细胞介素 4 (IL - 4 )的方法。用异硫氰酸荧光素 (FITC)和碱性磷酸酶(AP)分别标记识别不同表位的两种抗IL - 4单克隆抗体 (mAb) ,用抗FITC多抗或mAb包被免疫磁珠。结果显示 :检测标准品浓度在 0 - 3ng/mL范围内线性关系良好 ,敏感性达 10pg/mL ,平均回收率为 10 1.7% ,批内批间CV均为 3.6 % ,与ELISA比较相关系数为 0 .912 4。此法所需样本量少 ,方法稳定 ,敏感性高和特异性强 ,又无放射性污染 ,在临床检验和基础研究中有较大应用价值。In order to find a non-radioactive and sensitive method for detecting human interleukin 4(IL-4), a quantitative magnetic affinity immunoassay (MAIA) was established based on employing a pair of monoclonal antibodies against IL-4 with distinct epitopes and amplified system of FITC/anti-FITC mAb.The experimental condition, sensitive, precision, accuracy and the application of this method were evaluated.The linearity range of the MAIA measuring IL-4 was 0-3ng/mL, and the detectable limitation of IL-4 was 10pg/mL.The average recovery, intra-assay CV and inter-assay CV were 101.7%,3.6%,and 3.6% respectively.Compared with ELISA, the correlation coefficient was 0.9124.Taken together, the quantitative MAIA was established successfully with multi-advantages, which are stable, sensitive, reliable, less sample needed, without radioactive pollution and easy to perform.This method is worthy of recommendation in clinical and basic research application.

关 键 词：白细胞介素4 磁分离酶联免疫法 单克隆抗体 异硫氰酸荧光素 

分 类 号：R446.61[医药卫生—诊断学]