人瘦素放免分析法的建立及初步临床应用  

Development of Radioimmunoassay for Human Leptin and Its Primary Clinical Application

作　　者：赵明辉[1] 张春明[1] 王彦[1] 王德芝[1] 韩佩珍[1] 

机构地区：[1]中国医学科学院中国协和医科大学放射医学研究所,天津300192

出　　处：《标记免疫分析与临床》2004年第4期231-234,共4页Labeled Immunoassays and Clinical Medicine

摘　　要：瘦素是由肥胖基因编码 ,由脂肪细胞合成和分泌的一种亲水性激素蛋白。本文用高纯的重组人瘦素抗原免疫家兔 ,得到高效价的兔抗人瘦素多克隆抗体 ,氯胺 -T法制备12 5I-瘦素标记物 ,建立人血清瘦素RIA。本方法特异性强 ,灵敏度较高 ,精密度较好 ,其测定值与Linco公司的放免药盒有很好的一致性。用本法建立的正常参考值 (BMI 18- 2 5 ) :男性 (34例 )为 2 .15± 1.4 6ng/mL ,女性 (36例 )为 7.86± 3.6 0ng/mL。将 112名健康者的检测结果分成男、女两组 ,血清瘦素值与BMI之间有明显的正相关 (r=0 .72 ,P <0 .0 0 1)。瘦素水平与年龄之间无明显相关性 (P >0 .0 5 )。女性的促黄体激素、促卵泡激素、泌乳素、孕酮和雌二醇与瘦素无明显相关性 (P >0 .0 5 )。而对于男性 ,睾酮与瘦素水平成明显的负相关 (r=0 .792 ,P <0 .0 1)。Leptin was a hormone synthesized and secreted by lipid cells which encoded and expressed by the obese gene.In this article, rabbit-anti-human leptin polyclonal antibodies(PcAb) was prepared by immunizing rabbits with recombined human leptin while 125 I-leptin was obtained by chloramines-T method.A method of RIA for human leptin was developed, using these self-made preparations.The result of tests showed high sensitivity and specificity with reliable reproducibility, which proved that the quality of our preparations was no less good than that of the imported Linco product.The normal range(BMI 18-25) was 2.15±1.46ng/mL(male, 34cases) and 7.86±3.60ng/mL(female, 36 cases).There was significant positive correlation(r=0.72, P<0.001)between serum leptin concentrations and body-mass-index(BMI), in the case of 112 healthy persons were separated to male group and female group.Tere was no significant correlation between serum leptin concentrations and ages.So did follicle-stimulating hormone, luteinizing hormone, prolactin, progesterone and estradiol in females.But in males, serum leptin concentrations correlated directly and negatively with testosterone concentrations.

关 键 词：瘦素 多克隆抗体 放射免疫分析 

分 类 号：R817.4[医药卫生—影像医学与核医学]