辣椒内生菌拮抗细菌的筛选  

Isolation of pepper endophyte antagonists against Ralstonia solanacearum

作　　者：黎起秦[1] 卢继英[1] 林纬[1] 陈永宁 卢亭君 谢义灵[1] 韦文亮[1] 

机构地区：[1]广西大学农学院,广西南宁530005 [2]广西南宁市植保植检站,广西南宁530001

出　　处：《广西农业生物科学》2004年第4期304-306,共3页Journal of Guangxi Agricultural and Biological Science

基　　金：广西自然科学基金资助项目(桂科基0009018)

摘　　要：从广西一些县市采集辣椒茎标本,经分离得到41个细菌菌株,分属为土壤杆菌(Agrobacteriumspp.)、分枝杆菌(Mycobacteriumspp.)、微杆菌(Microbacteriumspp.)、欧文氏菌(Erwiniaspp.)和芽孢杆菌(Bacillusspp.),其中芽孢杆菌(Bacillusspp.)为优势种群。用抑菌圈法测定对茄青枯病菌有拮抗作用的有3个菌株,用浸种法和注射法测定具内生性的有17个菌株,具内生性同时又具拮抗作用的有2个菌株。Forty-one bacterial strains were isolated from the stems of pepper plants collected from some cities(counties) of Guangxi at the fruiting stage. The strains were preliminarily classified as Agrobacterium spp.,Mycobacterium spp., Microbacterium spp.,Erwinia spp.and Bacillus spp..Among these five isolated genera, isolates of Bacillus were the dominant endophytic bacteria in pepper plants. According to the test of inhibiting the growth of Ralstonia solanacearum,three strains have antagonistic activity against the plant pathogen. On the basis of the test of endo-growth in plant with the method of soaking seed and injecting the strain to the stem of pepper, seventeen strains were endomicrobite and two of them also inhibit the growth of R. solanacearum.

关 键 词：辣椒 内生菌 拮抗作用 茄青枯病菌 

分 类 号：S436.412.15[农业科学—农业昆虫与害虫防治]