非甲非戊型肝炎患者TT病毒感染的检测  

Detecting and sequencing TT virus DNA in patients with non A-E hepatitis

作　　者：何忠平[1] 周育森[2] 

机构地区：[1]北京地坛医院,100011 [2]军事医学科学院微生物流行病学研究所

出　　处：《临床输血与检验》2001年第1期6-8,共3页Journal of Clinical Transfusion and Laboratory Medicine

摘　　要：目的 了解非甲非戊型肝炎患者ＴＴ病毒感染状况。方法 采用套式ＰＣＲ方法对４４份临床诊断为急慢性非甲非戊型肝炎患者的血清标本，进行ＴＴ病毒检测。结果１４例ＴＴＶ－ＤＮＡ阳性，检出率３１．８％（１／４４）。对ＴＴＶ－ＤＮＡ阳性标本的ＰＣＲ产物进行克隆后测序，结果表明该序列与日本株相应位置核苷酸序列同源性为９８％。结论 推测ＴＴ病毒可能为临床急慢性非甲非戊型肝炎病因之一。Objective To study the infectious state of TT virus in patients with non A-E hepatitis. Method Fourty-four serum samples were detected for TTV-DNA using nested-PCR method. Results The positive rate was 31.8% (14/44). Two products of PCR were purified and cloned to T vector. The sequence analysis showed that there were above 98% nucleotide identity among AB008394 (from Japan) and 2 isolates from the patients. Conclusion The results suggest that there exist TTV infection among patients in Beijing region. TTV may be one of etiology in patients with acute or chronic non A-E hepatitis.

关 键 词：戊型肝炎 患者 TT病毒感染 TTV-DNA 慢性 血清标本 阳性标本 PCR产物 克隆 测序 

分 类 号：R512[医药卫生—内科学] R446[医药卫生—临床医学]