库血HCV-RNA的检测分析  

Detection and analysis of HCV-RNA In banked blood

作　　者：姚萍[1] 伍继新[1] 胡兆平[1] 陶良军[1] 叶冬青[2] 黄芬[2] 

机构地区：[1]安徽省立医院输血科,合肥230001 [2]安徽医科大学,合肥230001

出　　处：《临床输血与检验》2001年第1期9-10,共2页Journal of Clinical Transfusion and Laboratory Medicine

摘　　要：目的 为探索提高库血内型肝炎病毒（ＨＣＶ）的检出率。方法 采用逆转录套式聚合酶链反应（ＲＴ－ＰＣＲ）技术，检测４ ５３０份抗－ＨＣＶ阴性的库血ＨＣＶ－ＲＮＡ。结果 ４ ５３０份库血中ＨＣＶ－ＲＮＡ阳性５５份，阳性率１．２１％，其中个体献血者血液２ １１０份，ＨＣＶ－ＲＮＡ阳性４０份，阳性率１．９０％；无偿献血者血液 ２ ４２０份，ＨＣＶ－ＲＮＡ阳性 １５份，阳性率０．６２％。结论ＲＴ－ＰＣＲ可用于检测库血ＨＣＶ－ＲＮＡ，以减少因输血造成ＨＣＶ感染。Objective To study on improvement of detection rate of hepatitis C virus in banked blood. Methods Nested reverse transcriptional-polymerase chain reaction (RT-PCR) was used to detect HCV-RNA in 4 530 shares of banked blood in which anti-HCV was negative. Results There were 55 shares of positive HCV-RNA in 4 530 with the positive rate of 1. 21%. Among them, there were 40 shares of positive HCV-RNA in 2 110 individual blood donors with the positive rate of 1. 90% ,and 15 shares of positive 1-IC V-RNA in 2 420 volunteering blood donors with the positive rate of 0. 62%. Conclusions RT-PCR can be used to detect HCV-RNA in banked blood to reduce HCV infection because of blood transfusion.

关 键 词：HCV-RNA 库血 阳性率 血液 RT-PCR 检测分析 抗-HCV阴性 探索 目的 个体 

分 类 号：R512[医药卫生—内科学] R446[医药卫生—临床医学]