IRM-2近交系小鼠的遗传监测  

Genetic Monitoring of the IRM-2 Inbred Strain Mouse

作　　者：周继文[1] 王月英[1] 刘双环[2] 岳井银[1] 赵忠萍[1] 张荷清[1] 吴红英[1] 刘鹏[1] 穆传杰[1] 

机构地区：[1]中国医学科学院中国协和医科大学放射医学研究所,天津300192 [2]中国药品生物制品检定所

出　　处：《中国实验动物学杂志》2000年第4期206-209,共4页Chinese Journal of Laboratory Animal Science

基　　金：国家九五重点项目!(96-A23-06-04)

摘　　要：目的观察ＩＲＭ－２小鼠基因纯合性和遗传质量。方法用电泳法对ＩＲＭ－２近交系小鼠进行了生化标记基因检测，并做了毛色基因和皮肤移植试验。结果分析测定了第２３代小鼠１３条染色体上２５个基因位点和第３８代的１３个生化标记基因，所查基因位点全部纯合；同系异体间背部皮肤移植１００ｄ后，未见排斥现象，为同系组织遗传性；与ＢＡＬＢ／ｃ白化系小鼠交配进行的毛色基因测试，杂交第一代小鼠的毛色与ＩＲＭ－２小鼠一致，均为浅桂皮色，基因型为ＡＡｂｂＣＣＤＤ。结论可以认为，ＩＲＭ－２小鼠是遗传上高度纯合的近交系小鼠。Objective The aim of this study is to examine the genetic characteristics of the IRM-2 mouse which is a new inbred strain mouse hybridized by the ICR(paternal strain) with 615(maternal strain) and has progressed to 38th generation since 1990. Methods Genetic monitoring of the IRM-2 inbred strain mouse was performed by skin grafting test, coat color test and biochemical marker analysis. Results The skin grafting test showed that no dropping graft was found during 100 days after transplanting, implying the IRM-2 was of isohistogenicity. Analysis of coat color genes indicated that hair color of first generation hybrid crossed between F38 and BALB/c was cassia bark as same as the IRM-2, suggesting that the coat color genes of the IRM-2 of were homogenous. The gene type is AAbbCCDD. Measuring of 25 and 13 biochenmical markers gene loci on 13 chromosomes was performed by electrophoresis for F23 and F38 respectively. The results revealed that all observed gene loci were homogenous without any polymorphism and mutation. Conclusion The IRM-2 inbred strain mouse is high homogenous in the genetic characterishcs.

关 键 词：近交系小鼠 皮肤移植 遗传监测 基因检测 结果分析 BALB/C 观察 毛色 生化标记 基因位点 

分 类 号：Q78[生物学—分子生物学] R622.1[医药卫生—整形外科]