IRM-2近交系小鼠的血液学及血清生物化学研究  

Studies on Hematology and Serum Biochemistry of Inbred Strain Mouse IRM-2

作　　者：吴美羔[1] 周继文[1] 刘双环[2] 岳秉飞[2] 王月英[1] 吴红英[1] 岳井银[1] 张荷清[1] 穆传杰[1] 

机构地区：[1]中国医学科学院中国协和医科大学放射医学研究所,天津300192 [2]中国药品生物制品检定所

出　　处：《中国实验动物学杂志》2000年第4期237-239,共3页Chinese Journal of Laboratory Animal Science

基　　金：国家九五攻关项目(96-A23-06-04)

摘　　要：目的测定正常成年近交系小鼠 ＩＲＭ－２的一些血液学和血清生物化学参数。方法用自动光电血球计数仪测定分析了ＩＲＭ－２小鼠的血细胞成分及相关参数；用半自动生化分析仪测定了血清常用的１３项生化指标；并进行了常规法骨髓细胞的分类。结果雌、雄小鼠各１０只，平均生化指标及活度分别为：ＡＬＴ：３９．１Ｕ／Ｌ和４４．１Ｕ／Ｌ．ＡＳＴ：１２８．２ Ｕ／Ｌ和１１３．０ Ｕ／Ｌ，ＡＬＰ：１１７．７ Ｕ／Ｌ和７６．５Ｕ／Ｌ，ＧＬＵ：４．２ ｍｍｏ１／Ｌ和４．７ ｍｍｏ１／Ｌ， ＴＧ：０．７９ ｍｍｏ１／Ｌ和 ０．７３ ｍｍｏ１／Ｌ， ＣＨＯ：２．９ ｍｍｏ１／Ｌ和 ３．５ ｍｍｏ１／Ｌ， ＴＰ：２９．４ ｇ／Ｌ和 ２８．１ｇ／Ｌ， ＡＬＢ：２１．２ ｇ／Ｌ和 １８．９ ｇ／Ｌ， ＢＵＮ：５．２９ ｍｍｏ１／Ｌ和 ５．０ ｍｍｏ１／Ｌ， ＣＲＥ：Ｓ２．１μｍｏ１／Ｌ和５１．０μ ｍｏ１／１，Ｃａ：６．８ｍｇ／ｄ１和７．０ｍｇ／ｄ１， Ｐ：７．４ｍｇ／ｄ１和７．７ｍｇ／ｄ１，ＴＢＩＬ：３８ μｍｏ１／Ｌ和 ３８ μ ｍｏ１／Ｌ。外周血检测为ＲＢＣ：９．５ × １０１２／Ｌ和９．１× １０１２／Ｌ， ＷＢＣ：１０．３ × １０９／Ｌ和 ９．５ × １０９／Ｌ。Objective The aim of this study is to obtain some parameters of hematology and biochemistry in inbred strain mouse IRM-2. Methods 13 serum biochemistry parameters were measured using semi-auto chemistry analyzer (Vialad micro-95, Holland). The peripheral blood cell count and leucocyte differential count were analyzed by electrophotocounter for blood cell (MEK-5l08, Japan). The myelogram was examined on regular method. Results The average contents and activities of 13 biochemistry parameters from 10 female mice and 10 male mice were respectively as follows: ALT 39.1 U/L and 44.l U/L, AST 128.2 U/L and 113.0 U/L, ALP 117.7 U/L and 76.5U/L, GLU 4.2 mmol/L and 4.7 mmol/L, TG 0.79 mmol/L and 0.73 mmol/L, CHO 2.9 mmol/L and 3.5 mmol/L, TP 29.4 g/L and 28.1 g/L, ALB 21.2 g/L and 18.9 g/L,BUN 5.29 mmol/L and 5.0 mmol/L, CRE 52. 1 mol/L and 51 u mol/L, Ca 6.8 mg/dl and 7.0 mg/dl, P 7.4 mg/dl and7.7 mg/dl, TBIL 38 μmol/L and 38 μmol/L. Examination of peripheral blood cells revealed:f RBC 9.5 x 1012/L and 9.1 x 1012/L, WBC 10.3 x 109/L and 9.5 x 109/L, PLT 485 x 109/L and 604 x 109/L. Leucocyte differential count: 21 .2% and 31 .7% neutrophilic granulocytes (including both band-form and segmented), 78.7% and 68.2% lymphocytes for female and male respectively. Bone marrow differential count showed 45% granulocyte system and 22% erythrocyte system. Conclusion The parameters of hematology and biochemistry in inbred mouse IRM-2 are relatively stable, and the IRM-2 mouse as aniamal models of radio biology and radiotherapy would be available.

关 键 词：IRM-2小鼠 血液学 血清生物化学 

分 类 号：Q95-331[生物学—动物学] R446[医药卫生—诊断学]