嗜肺巴斯德杆菌选择性培养基研制及应用  

The Formulation and Application of a Selective Medium for Identification of Pasteurella pneumotropica

作　　者：李红[1] 刘星[1] 张丽芳[1] 许虎峰[1] 

机构地区：[1]中国医学科学院中国协和医科大学实验动物研究所,北京100021

出　　处：《中国实验动物学报》2001年第1期20-25,共6页Acta Laboratorium Animalis Scientia Sinica

基　　金：北京市科委实验动物科学专项基金资助!( 953991 2 0 0 )

摘　　要：目的　研制一种对嗜肺巴斯德杆菌表现出强选择作用的选择性培养基 ,用于该菌的常规检测。方法　药敏试验及抗生素最小抑菌浓度测定。结果　研制了嗜肺巴氏杆菌选择性培养基 (PPSM培养基 )及嗜肺巴斯德杆菌增菌液 (PP肉汤 )。嗜肺巴斯德杆菌在PPSM培养基上 ,37℃ 48h培养 ,形成 1mm左右 ,凸起、湿润、灰黑色并有金属光泽的特殊菌落 ;对表皮葡萄球菌和大肠埃氏菌的抑制率为 1 0 0 % ,对变形杆菌的抑制率为 76% ,并能抑制其迁徙生长 ;通过PP肉汤增菌培养 ,PPSM培养基使SPF小鼠粪便中嗜肺巴斯德杆菌检出率从 0增至 67 2 % ;用小鼠咽拭子接种该培养基 ,其初代培养物几乎为纯培养物。结论　该培养基对嗜肺巴氏杆菌具有较强的选择作用 ,使用该培养基对嗜肺巴氏杆菌进行检测可以简化检测程序、防止漏检。Objective To develop a selective medium (PPSM) that shows strong selection to P pneumotropica in conventional diagnosis Methods Drug sensitivity test and MIC were used Results A selective medium(PPSM) and its selective broth(PP broth) for Pasteurella pneumotropica were developed After incubation on PPSM at 37℃ for 48 hr,P pneumotropica formed special colony about 1mm in diameter which was convex,damp,grey or black with the luster of metals Its inhibitory rate to Staphylococcus epidermidis and E coli was 100%,to Proteus sp was 76%,and the characteristic swarming growth was inhibited After incubation in PP broth,PPSM made the positive rate of feces from SPF mice increased from 0 to 67 2% Primary culture was almost pure when mice laryngopharyngeal swabs inoculated on PPSM Conclusion The medium was strongly selective to P pneumotropica It could be very easy and correct for the diagnosis of P pneumotropica without sacrificing animals

关 键 词：嗜肺巴氏杆菌 小鼠 巴斯德 肉汤 咽拭子 表皮葡萄球菌 抑制率 选择性培养基 初代培养 纯培养 

分 类 号：Q93[生物学—微生物学] S858[农业科学—临床兽医学]