HRP法对异种神经移植后再生纤维恢复的形态学研究  

Morphological Study on the Nerve Fiber Regeneration after Heterograft by HRP

作　　者：何仲义[1] 焦旭文[1] 秦毅[1] 王效军[1] 牛建国[1] 

机构地区：[1]宁夏医学院解剖学教研室,银川750004

出　　处：《中国实验动物学报》2001年第3期139-142,共4页Acta Laboratorium Animalis Scientia Sinica

基　　金：宁夏回族自治区教育厅资助课题 (9710 2 92 0 2 )

摘　　要：目的　用辣根过氧化酶 (HRP)逆行追踪技术探讨异种神经移植后神经纤维的再生。方法　将多次冻融处理后的兔胫神经移植于大鼠坐骨神经 ,术后第 2、4、6、8和 10周 ,将HRP注入大鼠坐骨神经吻合部远侧端。结果　移植术后第 4周起在L4～ 5脊神经节见到HRP标记细胞 ,从第 6周在腰段脊髓前角内见到标记细胞 ,其数量随术后存活期延长而增多。术后 4周在移植神经内见少量再生神经纤维 ,6周后再生神经纤维穿过异种移植神经进入大鼠坐骨神经远侧端。结论　自移植术后 4周起 ,移植神经内已有再生纤维并部分恢复了轴浆流 。Objective To study the nerve fiber regeneration after heterograft with HRP retrograde tracing. Methods The tibial nerve of rabbit treated by repeated freezing and thawing for many times was grafted to rat sciatic nerve. At 2, 4, 6, 8 adn 10 weeks after grafting, HRP was injected into rat sciatic nerve distal. Results HRP labelled neurons were found in L 4～5 spinal ganglia at 4 weeks after grafting and also in anterior horn of the spinal cord at six. The number of the labelled neurons increased with prolonged survival time. A few regenerated nerve fibers were seen in the grafting nerve at 4 weeks and they passed through grafting nerve into distal part of the rat sciatic nerve at 6 weeks. Conclusions There were regenerated nerve fibers in grafting nerve and axoplasm flow of regenerated nerve fibers was recovered at 4. The present work proved that regeneration of the nerve fibers can be reflected by HRP.

关 键 词：术后 HRP 坐骨神经 异种 神经移植 大鼠 移植术 辣根 逆行追踪 数量 

分 类 号：Q554[生物学—生物化学] R651[医药卫生—外科学]