重组人白细胞介素-1β对冷冻后小鼠桑椹胚体内外发育率的影响  

Effect of Recombinant Human Inter Lenkin-1β(rhIL-1β) on the Development of Mouse Frozen-thawed Morulae

作　　者：朱士恩[1] 李玉欣[1] 曾申明[1] 张忠诚[1] 

机构地区：[1]中国农业大学动物科技学院

出　　处：《中国实验动物学杂志》1999年第2期65-68,共4页Chinese Journal of Laboratory Animal Science

摘　　要：本试验探讨了重组人白细胞介素－１β（ｒｈＩＬ－１β）对小鼠桑椹胚玻璃化冷冻后体内外发育的影响。结果表明：当ｒｈＩＬ－１β添加浓度为５、５０、５００ＩＵ／ｍｌ时，对解冻后的胚胎经体外培养，发育率（８７％～１００％）与对照组（９４％）相比无显著性差异（Ｐ＞００５）。而浓度提高到１０００ＩＵ／ｍｌ时，胚胎的发育受到抑制。解冻后胚胎在含有５、５０、５００ＩＵ／ｍｌ浓度的ｒｈＩＬ－１β培养液中培养３～４ｈ后移植于假妊娠３ｄ的受体鼠，移植妊娠率（８６％、１００％、８３％）高于对照组（５６％），特别是５０ＩＵ／ｍｌ处理组与对照组有极显著差异（Ｐ＜００１）。而浓度为１０００ＩＵ／ｍｌ处理组的胚胎移植妊娠率（３０％）低于对照组。试验证明，添加浓度为５～５００ＩＵ／ｍｌｒｈＩＬ－１β的培养液，对解冻后胚胎的体外发育率无影响，而且能够显著提高移植妊娠率。The present study examined effects of rhIL-1 β on the development of mouse froze n-thawed morulae. The frozen-thawed embryos were cultured in mKRB supplemented w ith 5IU/ml, 50IU/ml and 500IU/ml rhIL-1β, the rates of development were 87%, 97 % and 100% respectively. In comparisons between the presence or absence of rhIL- 1β, no differences were observed in the development rates. Further increase fro m 500IU/ml to 1000IU/ml rhIL-1β reduced the development rate. The frozen-thawed morulae were cultured in mKRB supplemented with 5IU/ml, 50IU/ml and 500IU/ml fo r 3～4h, then transferred into 3-day pseudopregnant recipients. The pregnant rat es in three groups were significantly higher than that in control. However, whe n the concentration of rhIL-1β increased to 1000IU/ml, the pregnant rate was si gnificantly lower than that in control. The results show that the presence of rhIL-1β in mKRB with the concentration from 5IU/ml to 500IU/ml had no effect on the devel opment of mouse frozen-thawed morulae in vitro, but it improved the pregnant rat es after the embryos were transferred into recipients.

关 键 词：对照组 IL-1β 体内 胚胎 小鼠 发育 白细胞介素-1Β 桑椹胚 移植妊娠率 解冻 

分 类 号：Q813[生物学—生物工程] S823[农业科学—畜牧学]